虹鳟卵母细胞体外成熟与授精的研究
STUDIES ONIN VITRO MATURATION AND FERTILIZATION OF RAINBOW TROUT OOCYTES
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摘要: 随着基因工程技术的发展,很多学者正致力于转基因鱼的研究。就实验材料而言,鱼类有体外受精、发育,怀卵量多、卵径大、便于显微操作等优点。然而鱼类受精卵的卵黄多、核很小,镜下难以分辨。特别是虹鳟鱼卵膜厚而不透明不可能将外源基因精确地注入到动物极内。因此转化率很低,只有通过大量地注射鱼卵来增加转化个体的数量。这不但要饲养大量实验鱼,而且为受体鱼的检测带来了很大的困难。一般来说通过体外培养成熟的卵母细胞膜薄而透明,核膨大明显可见。因此可以精确地将外源基因注入核区域内从而可以大大地提高转化率。
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Keywords:
- Rainbow trout /
- Oocyte /
- Maturation /
- Fertilization /
- invitro
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[1] Shi L Y, Studies on maturation and fertilization of red remora oocyte in vitro [J].Journal of fishery sciences of china, 1995,2(5):36-39.[石连玉.红鲫卵母细胞体外成熟和受精的研究.中国水产科学,1995,2(5):36-39][2] Zheng R Z,Rescarch on the extevnal maturation and fertilization of the oocyte of the Rabbit [J].Acta zoologica sinica,1992,38(1):64-71[郑瑞珍.兔卵母细胞体外成熟和体外受精的研究.动物学报,1992,38(1):64-71][3] Ji W Z,External maturation, fertilization and cultivation of the monkey s oocyte [J].Chinese Science Bulletin, 1992,18:2090-2092.[季维智.猕猴卵母细胞的体外成熟,受精和培养.科学通报,1992,18:2090-2092][4] Wang R X, Research to get the transgenic goldfish from the external maturated oocyte [J]. Chinese Science Bulletin,1990,18:1422-1424.[王壬学.从体外成熟卵母细胞获得转基因金鱼的研究.科学通报1990,18:1422-1424][5] Zhao W X,Changes of the sex steroid content in Hypophthalmichthys molitrix during its spawning [J].Acta Hydrobiologica Sinica,1988,12(3):212-216.[赵维信.诱导鲢排卵时性类固醇激素含量的变化.水生生物学报,1988,12(3):212-216][6] Zhao W X, Improvement of the measarement methods and the anual Changes of the esrtradial content in Megalo brama am blyocephala serum [J].Acta Hydrokiologica sinica,1987,11(2):97-104.[赵维信等.团头鲂血清中雌二醇含量测定方法的改进和周年变化规律的研究.水生生物学报,1987,11(2):97-104][7] Jiang R X,Variation of gonadotropin and 17β-esrtradial by LHRH-Ain Megalobrama ambly cephala [J].Journal of Fisheries of China,1986,10(2):187-193.[姜仁良.促黄体生成素释放激素类似物对团头鲂血清中促性腺激素和17β-雌二醇含量变动的研究.水产学报,1986,10(2):187-193][8] Zhao W X,Separating of ovarian follicle releasability steroids in Atlantic Salmon during its vitellogenic stages induced with gonadotropin [J].Journal of Fisheries of China, 1986,10(4):389-394.[赵维信.促性腺激素诱发大西洋鲑卵黄发生期卵巢滤泡释放性类固醇激素的离体研究.水产学报,1986,10(4):389-394][9] Wang Y Q, Fishery physiology [M].Shanghai:Science-technology Publisher of Shanghai, 1990,229-255.[王义强.鱼类生理学.上海:上海科学技术出版社,1990,229-255][10] Jaiabert B. Fostier A. The modulatory effect in vitro of oestradiol-17β testosterone or cortisol on the output of 17α-hydroxy-20β-dihydroprogesterone by rainbow trout (Salmo gairdneri) ovarian follicles stimulated by the maturational gonadotropin s-GtH [J]. Reprod. Nutr. Develop.1984,24(2):127-136[11] Barry T P, ;procarione L S,lapp A F et al. Induced final oocyte maturation and spawning in walleye (Stizostedion vitreu)[C].Aquaculture 1992,92:21-25 Shi L Y, Studies on maturation and fertilization of red remora oocyte in vitro [J].Journal of fishery sciences of china, 1995,2(5):36-39.[石连玉.红鲫卵母细胞体外成熟和受精的研究.中国水产科学,1995,2(5):36-39][2] Zheng R Z,Rescarch on the extevnal maturation and fertilization of the oocyte of the Rabbit [J].Acta zoologica sinica,1992,38(1):64-71[郑瑞珍.兔卵母细胞体外成熟和体外受精的研究.动物学报,1992,38(1):64-71][3] Ji W Z,External maturation, fertilization and cultivation of the monkey s oocyte [J].Chinese Science Bulletin, 1992,18:2090-2092.[季维智.猕猴卵母细胞的体外成熟,受精和培养.科学通报,1992,18:2090-2092][4] Wang R X, Research to get the transgenic goldfish from the external maturated oocyte [J]. Chinese Science Bulletin,1990,18:1422-1424.[王壬学.从体外成熟卵母细胞获得转基因金鱼的研究.科学通报1990,18:1422-1424][5] Zhao W X,Changes of the sex steroid content in Hypophthalmichthys molitrix during its spawning [J].Acta Hydrobiologica Sinica,1988,12(3):212-216.[赵维信.诱导鲢排卵时性类固醇激素含量的变化.水生生物学报,1988,12(3):212-216][6] Zhao W X, Improvement of the measarement methods and the anual Changes of the esrtradial content in Megalo brama am blyocephala serum [J].Acta Hydrokiologica sinica,1987,11(2):97-104.[赵维信等.团头鲂血清中雌二醇含量测定方法的改进和周年变化规律的研究.水生生物学报,1987,11(2):97-104][7] Jiang R X,Variation of gonadotropin and 17β-esrtradial by LHRH-Ain Megalobrama ambly cephala [J].Journal of Fisheries of China,1986,10(2):187-193.[姜仁良.促黄体生成素释放激素类似物对团头鲂血清中促性腺激素和17β-雌二醇含量变动的研究.水产学报,1986,10(2):187-193][8] Zhao W X,Separating of ovarian follicle releasability steroids in Atlantic Salmon during its vitellogenic stages induced with gonadotropin [J].Journal of Fisheries of China, 1986,10(4):389-394.[赵维信.促性腺激素诱发大西洋鲑卵黄发生期卵巢滤泡释放性类固醇激素的离体研究.水产学报,1986,10(4):389-394][9] Wang Y Q, Fishery physiology [M].Shanghai:Science-technology Publisher of Shanghai, 1990,229-255.[王义强.鱼类生理学.上海:上海科学技术出版社,1990,229-255][10] Jaiabert B. Fostier A. The modulatory effect in vitro of oestradiol-17β testosterone or cortisol on the output of 17α-hydroxy-20β-dihydroprogesterone by rainbow trout (Salmo gairdneri) ovarian follicles stimulated by the maturational gonadotropin s-GtH [J]. Reprod. Nutr. Develop.1984,24(2):127-136[11] Barry T P, ;procarione L S,lapp A F et al. Induced final oocyte maturation and spawning in walleye (Stizostedion vitreu)[C].Aquaculture 1992,92:21-25
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