Abstract: In order to optimize the breeding model and improve the breeding conditions, the early behavior characteristics of Glyptosternum maculatum larvae and juveniles, such as gap, bottom color and bottom species selection, were studied. The results showed that the preference of the Glyptosternum maculatum larvae and juveniles for gaps was significantly higher during the day (7:00—20:00) than that in night (21:00—6:00) (P<0.05). There were no preference for gaps (P>0.05) in feeding days on the 22th, 23th and 25th—30th, and no preference for gaps (P>0.05) at night (except feeding days on the 6th, 11th—13th). On the 12th, 13th, 15th and 24th day of feeding, the larvae and juveniles showed preference for smaller gaps (0.9 cm) (P<0.05); On the 2nd and 3rd days of feeding, the larvae and juveniles showed preference for bottom gaps (P<0.05); On the 5th, 6th, 8th, 21st and 24th days of feeding, the larvae and juveniles showed a preference for surface gaps (P<0.05); Under the light compensation of (400±50) lx, the preference of larvae and juveniles for the different background colors was not obvious; under the light compensation of (10±2) lx (P<0.05), the larvae and juveniles have a strong hiding behavior during the day to the small gap and they like black substrate under the condition of low light compensation. The hiding characteristics of juveniles and juveniles provide an important scientific basis for optimizing seedling cultivation and improving the environment of seedling cultivation.