溶藻细菌DC-L5的分离、鉴定及其溶藻特性

卢兰兰; 李根保; 沈银武; 胡明明; 刘永定

溶藻细菌DC-L5的分离、鉴定及其溶藻特性

中国科学院水生生物研究所, 武汉 430072
2. 中国科学院研究生院, 北京 100049

基金项目:

863课题(编号:2005AA60101504)

973项目(编号:2002CB412300)资助

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- 收稿日期: 2008-01-23
- 修回日期: 2008-10-28
- 发布日期: 2009-09-24

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF A STRA IN OF BLUE2GREEN ALGAE2LYS ING BACTERIUM FROM LAKE D IANCHI

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 从滇池蓝藻水华集聚区分离获得一株溶藻细菌DC-L5,通过形态及16S rDNA测序分析鉴定为短小芽孢杆菌。用小白鼠进行生物安全实验,小白鼠无中毒症状。研究表明当细菌处于对数生长期时溶藻效果最强,共培养5d使铜锈微囊藻的叶绿素a含量下降83.33%,使惠氏微囊藻、绿色微囊藻、水华束丝藻和水华鱼腥藻4种蓝藻叶绿素a下降率最高为67.6%,最低为58.5%,平均为62.25%。离心沉降后,发现沉淀菌体和无菌上清液对铜锈微囊藻都有溶藻效果,但溶藻效果不及原菌液,推测DC-L5可能是通过直接接触使藻细胞凝聚下沉及进一步的生物降解,同时存在抑制藻细胞生长的胞外分泌物。高温热处理后菌液溶藻作用不明显,推测高温可能使菌体或胞外分泌物质失活。
- 溶藻细菌 /
- 生物控藻 /
- 芽孢杆菌 /
- 蓝藻水华 /
- 微囊藻
Abstract: In this study,one high-effect algae-lysing bacterium was isolated and purified from Lake Dianchi,which wasabled as the strain DC-L5. The effect,host range,biosafety,lysing mechanism and lysing phenomenon of the algae-ly-ing bacteria were analyzed. Resultswere as the followings: The strain was identified asB acillus pum ilus bymeans ofmor-hological observation and 16S rDNA sequence analysis in the CCTCC (China Center for Type Culture Collection). Toxic-ty test with mouse primarily showed that the strain was non-toxic. 4 kinds of DC-L5 cultures of different growth phasesere added to M icrocystis aeruginosa,and the strongest algae-lytic ability was found in the log phase of DC-L5 cultures,hlorophyll a contents in culture ofM icrocystis aeruginosa reduced by 83. 33% in 5 days. For the host range experiment,kinds of dominant cyanobacteria in Lake Dianchi was researched,and average reduction rates of Chlorophyll a of-. -5% in cultures ofM icrocystis viridis,M icrocystis wesenbergii,Aphanizomenon flos-aquae and Anabaena flos-aquaewasbserved with the highest 67. 6% and the lowest 58. 5% in 4 days respectively. After centrifugation and sedimentation,oth the cells of bacteria and the cell-free suspension had strong algae-lytic activity,but the bacteria culture had the stron-est algae-lytic activity,so it maybe showed the strain DC-L5 could directly cause the cyanobacterial cells agglomerated,inked down and further lysed while the strain could excrete extra-cellular substances able to lyse cyanobacterial cells. Inrder to improve the algae-lytic activity of the strain DC-L5 to nature bloom,the bacteria cultures were added to natureyanobacterial bloom dominant with M icrocystis aeruginosa,which were samp led from Lake Dianchi and cultured in theab. The result of nature bloom experiment showed the strain DC-L5 firstly disaggregated the cyanobacterial colony,educed the floatability,degraded the p igments and then released inclusions of algal cells.
- Algaelysing bacterium /
- Biological control /
- B acillus /
- Water bloom /
- M icrocystis

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溶藻细菌DC-L5的分离、鉴定及其溶藻特性

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ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF A STRA IN OF BLUE2GREEN ALGAE2LYS ING BACTERIUM FROM LAKE D IANCHI

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