长吻鮠血细胞发生的研究

THE HEMATOCYTOGENESIS IN LEIOCASSIS LONGIROSTRIS

  • 摘要: 用Wright-Giemsa和PAS染色对长吻鮠头肾、肾脏、脾脏、肝脏等器官组织的涂片、印片染色观察发现, 头肾、肾脏和脾脏是其主要造血器官. 红细胞、粒细胞和淋巴细胞主要在肾脏和头肾中发生, 其次是脾脏. 单核细胞则主要在肾脏和脾脏中发生, 头肾中也有少量单核细胞产生. 肝脏中无原始型血细胞, 可能不是其造血器官. 红细胞的发育经历四个阶段, 其胞体体积经历了由大到小, 由小到大再变小的"两大两小"发育过程;粒细胞的发育经历五个阶段, 其胞体体积均由大变小, 双叶或多叶核的粒细胞可能是衰老的粒细胞亦即核的分叶是粒细胞衰老的标志;淋巴细胞和单核细胞的发育各经历了三个阶段, 两者发育成熟过程中胞体体积均由大变小. 巨噬细胞由单核细胞发育而来. 原血细胞和部分早期幼稚血细胞可以进行有丝分裂, 部分成熟红细胞和血栓细胞可以进行直接分裂. 红细胞在整个发育过程中, PAS反应均呈阴性, 各类白细胞的发育过程中, PAS反应由阴性到阳性并逐渐增强, 这显示随着白细胞的逐渐发育成熟, 细胞内糖原物质含量逐渐增多.

     

    Abstract: The Wrigh-t Giemsa and PAS stained imprints and smears of hematopoietic organs were observed by the light microscopy. The results showed that the head kidney, body kidney and spleen were the principal hematopoietic organs. Erythrocytes,lymphocytes and granulocytes developed mainly in head kidney and body kidney, and some of them developed in spleen.While most of monocytes developed in body kidney and spleen, some of them in head kidney. No primit ive blood cells were observed in liver smears, so liver was not a hematopoietic organ.The erythroid lineage consisted of proerythroblast, basophilic erythroblast, acidophilic erythroblast and erythrocyte. During maturation of the erythroid cells, two trends in size were distinguished: two decreasing and one increasing. Due to the great changes of granuloid cells in maturation, the granuloid lineages were divided into five stages: granuloblast, young granulocyte,progranulocyte, metagranulocyte and granulocyte. During the maturation of granuloid cells, they were reduced in size. The peripheral blood was the final place for their development. Maybe the bilobed granulocytes were ageing or senile granulocytes and the split of nucleus was a sign of senility. The lymphoid lineages were divided into three stages: lymphoblast, prolymphocyte and lymphocyte. The development of mononuclear cells was similar to that of lymphoid cells. They experienced three stages respectively: monoblast, promonocyte and monocyte. These two kinds of cells regularly reduced in size during development. Macrophages were derived from monocyte and they had phagocytosis. The primitive blood cells and some early immature blood cells all had mitotic activity. Some bilobed erythrocytes and bilobed thrombocytes could be observed and were considered to be cells in amitotic division. Precursors of thrombocytes were not encountered.During all the stages of the erythroid lineage, the erythroid cells were all PAS negative. While during the development of leucocytes, the cells were from negative to positive with PAS staining, and it suggested that with the maturing of the leucocytes the contents of glycogen were increasing gradually.

     

/

返回文章
返回