丁不同群体间形态学差异与随机扩增多态DNA(RAPD)分析
MORPHOLOGICAL VARIATION AND RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD)ANALYSIS OF DIFFERENT POPULATIONS OF TINCA TINCA
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摘要: 采用聚类分析、主成分分析和判别分析3种数理统计方法,对我国新疆朱家湖丁群体7、3水库丁群体和从捷克引进我国的丁群体的可量性状和框架参数进行分析。聚类分析和主成分分析显示,朱家湖群体与73水库群体较为接近,而捷克群体与前两群体相距较远。判别分析亦可将捷克群体与前两群体分开,准确率达100%。从60个随机引物中筛选出的20个引物对丁朱家湖群体、73水库群体和捷克群体进行了随机扩增多态DNA(RAPD)分析。引物S440在捷克群体扩增出的450bp片段为该群体特有的标记片段。朱家湖群体、73水库群体、捷克群体多态位点比例分别为24%、22.67%和18.42%;群体内遗传相似度分别为0.8967、0.9035和0.9309;遗传多态度(π)分别为0.1539、0.1489和0.1142。表明朱家湖群体保持着较高的遗传变异。三群体间的遗传相似度为0.6868-0.9496,群体遗传分化系数(Fst)为0.048-0.238,分子方差分析发现群体内方差占总方差的83.96%,群体间的方差只占16.04%,由此推断三群体间遗传分化并不大。
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关键词:
- 丁 /
- 遗传多样性 /
- 形态学 /
- 多态位点比例 /
- 随机引物扩增多态DNA
Abstract: The morphological and truss network characters of three populations of Tinca tinca, e.g., two indigenous populations from Zhujiahu Lake and 73 Reservoir of Xinjiang, China, and one exotic population from Czech, were studied by three multivari-ate analysis (cluster analysis, discriminant analysis and principal component analysis). The results showed that Zhujiahu popula-tion has a close relationship with 73 Reservoir population, and Czech population is far from other two populations.This was in ac-cord with the geographical distribution of the three populations. With 20 primers screened out from 60 primers, using RAPD(random amplified polymorphic DNA)technique, these three populations were analyzed.The proportions of polymophic loci of Zhujiahu Lake, 73 Reservoir and Czech were 24 %, 22. 67 % and 18. 42 %respectively.The genetic diversity index (л)was 0. 1539,0. 1489 and 0. 1142 respectively.These indicated that Zhujiahu population had higher genetic diversity than the others.The genetic similarity among three populations was 0. 6868-0. 9496.The fixation index (Fst)was 0. 048-0. 238. Variation within population accounted for 83. 96 %of total variation and variation between populations was only 16. 04 %of total variation.It was concluded, accordingly, that the genetic differention among three populations was low. -
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