短期饥饿胁迫对鮸鱼生化组成、脂肪酸和氨基酸组成的影响

BIOCHEMICAL COMPOSITION, AMINO ACID AND FATTY ACID COMPOSITION IN JUVENILE OFMIICHTHYSMIIUY UNDER SHORT-TIME STARVATION

  • 摘要: 在水温(22±1)℃条件下,研究了短期饥饿胁迫对鮸鱼幼鱼生化组成、脂肪酸和氨基酸的影响。幼鱼分别饥饿0d(S0)、饥饿3d(S3)、饥饿6d(S6)、饥饿9d(S9)、饥饿12d(S12)和饥饿15d(S15)。结果表明:鱼体内的粗蛋白、粗脂肪和糖类随着饥饿时间的延长而逐渐降低,而水分和灰分随着饥饿时间的延长而逐渐升高。饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)显著下降(p0.05);而多不饱和脂肪酸(PUFA)呈先上升趋势。鮸鱼幼鱼短期饥饿胁迫过程中,首先利用饱和脂肪酸,然后利用低不饱和脂肪酸,最后才动用高不饱和脂肪酸。双键位置不同的脂肪酸在饥饿过程中的损失速度也有差异,具体为n-9PUFAn-6PUFAn-3PUFA。S0主要氨基酸为天冬氨酸、谷氨酸、蛋氨酸、亮氨酸、赖氨酸和精氨酸。经过不同时间饥饿后,氨基酸总量和必须氨基酸总量显著下降,与S0组均差异显著(p0.05),但S9、S12和S15的基酸总量和必须氨基酸总量差异性不显著(p0.05)。

     

    Abstract: Food shortage is often a stress in natural environment to many fish species, caus ing growth retardation and even death.H owever, response to the stress differs in species.After the food becom e availab le again, they would re-ga in growth, which is called compensatory growth.Previous studies showed that compensatory growth could significant mi pact on biochemical composition, am ino acid and fatty acid composition.The main focus study reports of Miichthy smiiuy were that the artificial controlled techno logical research of gonad development, embryos and early larvae occurrence, brood-stock culture and artificial propagation, environm ental toxicology, and so on.To date, there had no research reports about the fish hunger phys iological ecology.The effects of short-time starvation on biochemical com pos ition, am ino acid and fatty ac id composition in the early breaded juvenile of Miichthy smiiuy was researched at(22±1)℃.The early breaded juveniles were divided into six groups(S0, S3, S6, S9, S12 and S15), which were starved for 0, 3, 6, 9, 12 and 15d respectively.The results show ed that themusclem oisture and ash in difference groups were significant difference compared with S0(p n- 6 PUFA> n-3 PUFA.The main amino acid of S0 was A spartic acid, G lutamic acid, M et, Leucine, arg inine and Lysine.After several different short-time starvations, both the total and the essential am ino acid reduced significantly, which were different with S0 group(p< 0.05).But there was insignificantly along with S9, S12 and S151

     

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