雨生红球藻营养细胞的虾青素累积
ASTAXANTHIN ACCUMULATION IN VEGETATIVE CELL OF HAEMATOCOCCUS PLUVIALIS
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摘要: 接种于缺氮培养基的雨生红球藻.当置于光强为10-12klx,温度25℃±1.5℃条件下培养时,营养细胞迅速由绿色变成红色.接种4d时,运动细胞占细胞总数的90%,细胞内的虾青素含量(33.0pg/cell)占最终色素累积量的(60.0pg/cell)的55%.接种6d时,虾青素含量已占最终累积量的75%.电镜观察显示,红色的运动细胞除细胞质的大部分区域充满色素颗粒外,细胞的形态结构与绿色细胞基本一致.实验还表明,当接种物置于低光(0.5-1.0klx)下培养时,营养细胞仍有色素沉积,但累积缓慢.当只有高光胁迫(10-12klx,完全培养基培养)时,营养细胞转变成厚壁孢子后(5d后),才大量积累色素.Abstract: When H. pluvialis was inoculated in a nitrogen-deficient medium and exposed to high light intensity condition(10—12 klx), the change of green motile cells to red motile cells was observed in four days. After four days of inoculation, the number of vegetative cells was 90% of total number of cells and the astaxanthin content(33.0 pg/cell)was 55% of the final astaxanthin content(60.0 pg/cell)which was inoculated ten days. After six days, the astaxanthin content was 75% of final astaxanthin content. It was found by electron microscopic observation that the cytoplasm of red motile cells was almost full of pigment granules, but the morphology and structure of cell was identical with green motile cells. The experiments also showed that the astaxanthin accumulation in motile cells would occur too when H. pluvialis was exposured to nitrogen starvation but lower light intensity condition(0.5—1.0 klx). When H. pluvialis was only exposured to high light-stress condition(inoculated in complete medium), the large amount pigment accumulation was only found during the transformation of vegetative cells to akintes.
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Keywords:
- Haematococcus pluvialis /
- Vegetative cell /
- Astaxanthin /
- Stress condition /
- Ultrastructure
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[1] Grung M D,Souza F M L,Burowitzka M,et al.Algal carotenoids 51.Secondary carotenoids Haematococcus pluvialis aphanospores as a source of(3S,3'S)-astaxanthin esters [J].J.Appl.Phycol.1992,4:165—171[2] 孙伟,王晓东译,微藻产品的经济价值及开发潜力[J].中国海洋药物,1995,2;48—52[3] Elliott A M.Morphology and life history of Haematococcus pluvialis Arch [J].Mikrobiol.1934,82:250—71[4] Lee Y K,Ding S Y.Cell cycle and accumulation of astaxanthin in Haematococcus lacustris [J].J.phycol.1994,30:445—449[5] 庄惠如,施巧琴,卢海声,等.营养胁迫对雨生红球藻虾青素累积的影响[J].水生生物学报,2000,24(3);208—212[6] 金传荫,宋立荣,刘永定,等.红球藻水生748株营养需求的研究[J].水生生物学报,1996,20(3);292—296[7] Boussiba S,Voushak S.Astaxanthin accumulation in the green alga Haematococcus pluvialis[J].Plant Cell Physiol,1991,32(7):1077—1082[8] Davies B H.Carotenoids.In;Chemistry and Biochemistry of Plant Pigments [M].London;Academic Press,1976,38—165[9] Santos M F,Mesquita J F.Ultrastructural Study of Haematococcus lacustris(Girad.)Rostafinski(Volvocales)I.Some aspects of carotenogensis[J].Cytologia,1984,49:215—228[10] Kobayashi M,Kakizono T,Yamaguchi K,et al.Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions[J].Journal of Fermentation and Bioengineering,1992,74(1):17—20[11] Yong Y Y R,Lee Y K.Do carotenoids play a photoprotective role in the cytoplasm of Haematococcus lacustris(chlorophyta)[J].Phycologia,1991,30(3):257—261 Grung M D,Souza F M L,Burowitzka M,et al.Algal carotenoids 51.Secondary carotenoids Haematococcus pluvialis aphanospores as a source of(3S,3'S)-astaxanthin esters [J].J.Appl.Phycol.1992,4:165—171[2] 孙伟,王晓东译,微藻产品的经济价值及开发潜力[J].中国海洋药物,1995,2;48—52[3] Elliott A M.Morphology and life history of Haematococcus pluvialis Arch [J].Mikrobiol.1934,82:250—71[4] Lee Y K,Ding S Y.Cell cycle and accumulation of astaxanthin in Haematococcus lacustris [J].J.phycol.1994,30:445—449[5] 庄惠如,施巧琴,卢海声,等.营养胁迫对雨生红球藻虾青素累积的影响[J].水生生物学报,2000,24(3);208—212[6] 金传荫,宋立荣,刘永定,等.红球藻水生748株营养需求的研究[J].水生生物学报,1996,20(3);292—296[7] Boussiba S,Voushak S.Astaxanthin accumulation in the green alga Haematococcus pluvialis[J].Plant Cell Physiol,1991,32(7):1077—1082[8] Davies B H.Carotenoids.In;Chemistry and Biochemistry of Plant Pigments [M].London;Academic Press,1976,38—165[9] Santos M F,Mesquita J F.Ultrastructural Study of Haematococcus lacustris(Girad.)Rostafinski(Volvocales)I.Some aspects of carotenogensis[J].Cytologia,1984,49:215—228[10] Kobayashi M,Kakizono T,Yamaguchi K,et al.Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions[J].Journal of Fermentation and Bioengineering,1992,74(1):17—20[11] Yong Y Y R,Lee Y K.Do carotenoids play a photoprotective role in the cytoplasm of Haematococcus lacustris(chlorophyta)[J].Phycologia,1991,30(3):257—261
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