Cd2+胁迫对石菖蒲生理生化特性的影响
EFFECTS OF ACORUS TATARINOWII ON PHYBIOCHEMICAL CHARACTERISTICS UNDER Cd~(2+) STRESS
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摘要: 通过水培实验,研究了不同浓度(0、50、200、400μmol/L)下和不同时间内(3、7、11d)Cd2+对石菖蒲(Acorus ta-tarinowiiSchott)叶片抗氧酶活性、叶绿素含量、脂质过氧化程度以及脯氨酸含量的影响。结果表明:(1)超氧化物歧化酶(SOD)活性在3d时随着胁迫浓度的增大而增加,但7d和11d时都是在50μmol/L浓度下达到峰值,然后降低,但都高于对照;除400μmol/L处理下SOD随胁迫时间延长呈先降后升的趋势外,其余浓度处理下均升高。(2)过氧化物酶(POD)活性在胁迫3d和7d时均随着胁迫浓度的增大而增加,11d时在200μmol/L处理下达到峰值,然后降低;各浓度处理组均随着胁迫时间的延长POD活性呈下降趋势。(3)过氧化氢酶(CAT)活性在不同时间内随着胁迫浓度的增加呈先增后降趋势,并且各浓度处理组的CAT均随胁迫时间的延长而增加。(4)叶绿素含量在不同胁迫时间内均在200μmol/L处理下达到峰值,然后降低;除400μmol/L处理下的叶绿素随胁迫时间延长而增加外,其余浓度处理组均呈下降趋势。(5)丙二醛(MDA)含量和脯氨酸(Pro)含量均随着胁迫浓度的增加和胁迫时间的延长而增加。研究结果分析表明石菖蒲对Cd2+胁迫有一定的耐受性。Abstract: The objective of this study was to investigate the Cd2+ stress on the changes of phybiochemical characteristics in Acorus tatarinowii,which would be helpful both to the research of biological function of Cd2+and the biotransformation of Cd2+ by A.tatarinowii.Different concentrations of Cd2+were added to A.tatarinowii and measured phybiochemical characteristics on the 3d,7d and 11d respectively.The effects of different Cd2+ concentrations stress and different stress times on antioxidant activities,chlorophyll contents,membrane lipid peroxidation and Pro content in the leaves of A.tatarinowii were investigated by water culture experiments.The results showed that:(1) The activity of SOD increased with the increasing of Cd2+concentration on 3d,which was up to the peak at 50 μmol/L of Cd2+concentration and then decreased on 7d and 11d,but it was higher than the control.Except it increased first and then decreased with the prolongation of Cd2+stress time under 400 μmol/L,other concentration treatments increased.(2) The activity of POD increased with the increasing of Cd2+concentration on 3d and 7d,which was up to the peak at -00 μmol/L of Cd2+concentration and then decreased on 11d.With the prolongation of Cd2+stress time,POD activity decreased in all concentration treatments.(3) The activity of CAT increased first and then decreased with the increasing of Cd2+concentration in different stress times and increased with the prolongation of Cd2+stress time in all concentration treatments.(4) Chlorophyll content was up to the peak at 00 μmol/L of Cd2+concentration and then decreased in different stress times.Except it increased with the prolongation of Cd2+stress time under 400 μmol/L,other concentration treatments decreased.(5) MDA content and Pro content increased with the increasing of Cd2+concentration and the prolongation of Cd2+stress time.The analysis results showed that Acorus tatarinowii had a certain tolerance to Cd2+.