鳡幼鱼肌纤维发育特征及驯食策略对肌肉发育的影响

DEVELOPMENT CHARACTERISTICS OF MUSCLE FIBER IN JUVENILE ELOPICHTHYS BAMBUSA AND THE EFFECTS OF WEANING STRATEGIES ON MUSCLE DEVELOPMENT

    摘要
  • 摘要: 为研究鳡(Elopichthys bambusa)幼鱼肌肉发育的分子机制, 通过组织学和转录组分析, 结合荧光定量PCR验证, 探讨不同发育阶段和驯食策略下鳡幼鱼肌肉的发育特征。结果表明, 鳡幼鱼肌纤维的马赛克增生从孵出后第22天(dph)开始, 相关肌源性调节因子基因(myog和myod)、肌肉生长抑制素基因(mstn)、肌卫星细胞标志基因(pax7)及肌肉分化相关基因(myl3、tnni2、myoz2、tpm1)在肌肉马赛克增生早期(15—22 dph)的表达出现显著性差异(P<0.05)。此外, 人工配合饲料可提升鳡幼鱼肌肉代谢水平促进其生长, 且22 dph开始驯食组具更高驯化效率和更良好的驯化效果。研究结果为理解鳡肌肉发育特征及其分子机制提供了基础, 并为规模化养殖提供理论参考。

     

    Abstract: In order to study the molecular mechanism of muscle development of juvenile Elopichthys bambusa, the characteristics of muscle development of juvenile Elopichthys bambusa under different developmental stages and domestication strategies were discussed by histological and transcriptome analysis and fluorescence quantitative PCR verification. The results showed that the mosaic proliferation of muscle fibers of Elopichthys bambusa larvae began on the 22nd day post-hatching (dph). During the early stage of muscle mosaic proliferation, genes related to MyoGenic regulators (myog and myod), the myostatin gene (mstn), muscle satellite cell marker gene (pax7), and muscle differentiation-related genes (myl3, tnni2, myoz2, tpm1) were actively involved. In addition, the use of artificial compound feed can improve muscle metabolism and promote growth in juvenile Elopichthys bambusa, with the 22 dph group showing higher weaning efficiency and better weaning effect. This study provide a foundation for understanding the characteristics and molecular mechanism of muscle development in Elopichthys bambusa and provide a theoretical reference for large-scale farming practices.

     

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