Abstract: Shellless hatching technology has been widely used in the field of bird artificial hatching, but it is rarely reported in reptiles. In this paper, the fertilized eggs of the Asian yellow pound turtle (Mauremys mutica) were used as the material, the embryo development of Mauremys mutica was observed in vivo and the whole process of embryo development was recorded. When the incubation temperature is (32±0.5)℃, the incubation period is 54d on average. Under the condition of shellless, without external water and oxygen, the embryos of Mauremys mutica can complete the whole process of development and successfully hatch young turtles. According to the morphological characteristics and time of embryonic development, the whole development process is divided into 19 stages. Stages 1—7 are based on the vascular plexus of the yolk sac, the shape and beating of the heart as staging criteria; and the main staging criteria for stages 8—19 are the head, limbs, back armor, color, and allantoic sac. This study demonstrates that the calcium source of the egg shell is not essential for embryonic development of turtles. The water content of the egg itself can meet the needs of the entire embryonic development process. Moreover, turtle embryos have extremely strong resistance to hypoxia. The successful application of shellless hatching technology in the observation of turtle embryo development shows that it has a broad potential application prospect. Shellless hatching technology is expected to become a new ideal experimental model. Based on the simple and convenient operation methods, turtle embryo models can be mass-produced for application in basic and applied scientific research fields.