Abstract: The present study collected samples of Pseudorasbora parva from the Lhalu Wetland and Chabalang Wetland in Tibet Autonomous Region from 2019 to 2021 and analyzed the life-history traits of the two populations. By comparing with populations from Nanjing in lower reaches of the Yangtze River and other low-elevation regions in the native range, we aimed to reveal its adaptation of life-history traits in the plateau. The results showed that the ages of P. parva in the Lhalu Wetland and Chabalang Wetland ranged from one to four years, with the dominant ages of one year and two years. The ages of P. parva in Nanjing ranged from one to five years, with the dominant ages of three years and four years. The relationships between body length (L) and body weight (W) were W_♂ =0.021 L^2.966(r²=0.982) and W_♀ =0.014 L^3.224(r²=0.986) in the Lhalu population, W_♂=0.011 L^3.282(r²=0.990) and W_♀=0.014 L^3.123(r²=0.989) in the Chabalang population, and W_♂=0.014 L^3.146(r²=0.939) and W_♀=0.028 L^2.763(r²=0.894) in the Nanjing population. The relationships between body length and scale radius (R) were L_♀=21.676 R+7.766 (r²=0.886) and L_♂=29.184 R^0.792(r²=0.886) in the Lhalu population, L_♀=16.946 R+15.048(r²=0.857) and L_♂=18.290 R+13.157(r²=0.858) in the Chabalang population, and L_♀=33.710 R^0.607(r²=0.686) and L_♂=16.194 R+20.485(r²=0.800) in the Nanjing population. The values of absolute fecundity and egg diameter were (926±476) and (0.92±0.10) mm in the Lhalu population, 364 and 0.97 mm in one female sample from the Chabalang population, and (1220±704) and (1.00±0.13) mm in the Nanjing population. Compared with native populations, the Lhalu and Chabalang populations possessed a lower age structure, lower values of average body length, smaller sizes and slower growth rates at each age group, and lower values of absolute fecundity and egg diameter. The cold condition may be the main factor resulting in changes in these life-history traits (e.g. slow growth and low fecundity) of P. parva in Tibet Autonomous Region.