Abstract: Personality refers to the persistent and consistent behavioral differences among individuals in a population under different spatio-temporal conditions. Predator-prey interactions are common in nature, and predation environment is the main driving force for the evolution of prey phenotypes (e.g. personalities). To investigate interspecific differences in the personalities of prey fish species and the effects of their predator, Channa argus was used as the predator, Rhodeus ocellatus and Pseudorasbora parva, which usually form mixed populations in nature, were used as the prey fish in this study. The prey fish were exposed to predator treatment or no predator treatment for one month, using the common garden experiment. Subsequently, the personalities, including exploration, activity and boldness of two prey fish species were measured, and the interspecific differences of different personalities and their environmental dependence were analyzed. The results showed that: (1) There were significant interspecific differences in personality between the two prey fish species. The exploration, activity and boldness of R. ocellatus were significantly stronger than those of the P. parva, regardless of whether they had undergone predator treatment or not (P<0.05). (2) Predator experience led to enhanced exploration and boldness of two prey fish species but had no significant effect on the activity of both (P>0.05). (3) Predator attenuated the interspecific differences of different personalities between two prey fish species and strengthened the coupling of their individual different personalities. The results suggested that the personalities of the prey fish have significant differences between species and both have a predator effect. Predator experience leads to the decrease of interspecific differences in the personalities of the two prey fish species, showing a trend of convergent adaptation.