Abstract: Animals can improve their feeding efficiency and preference through learning and memory. As a key factor of learning and memory, c-fos plays an important role in animal feeding behavior. In this study, the brain cells of Siniperca chuatsi were treated with the inhibitors of learning and memory pathway T-5224 and KN-62. It was found that T-5224 had a negative regulation effect on c-fos while KN-62 had a positive regulation effect on c-fos, and the appropriate treatment time and concentration were selected. The mRNA expression of c-fos and t1r1 in the brain of mandarin fish decreased significantly after being treated with 30 μmol/L T-5224 for 24 hours (P<0.05), and increased significantly after being treated with 200 nmol/L KN-62 for 2h (P<0.01). In addition, the methylation changes of t1r1 gene after inhibitor treatment were also analyzed. However, there was no significant change in the methylation level of t1r1 in the brain of Siniperca chuatsi (P>0.05). The above results indicated that the transcription level of taste receptor t1r1 in brain cells of Siniperca chuatsi may be regulated by learning and memory factor c-fos gene, while c-fos may regulate t1r1 transcription through other ways, but not DNA methylation. At the same time, it provides theoretical basis for the change of feeding preference of Siniperca chuatsi after domestication. In this study, it was confirmed for the first time that T-5224 negatively regulated the learning and memory factor c-fos, while KN-62 positively regulated it. At the same time, the above results indicated that the expression of c-fos, the key transcription factor of learning and memory, affected the expression of taste receptor t1r1, and the c-fos gene might participate in the feeding habits of Siniperca chuatsi.