Abstract: According to acoustic signals, biotelemetry can monitor the behavior patterns of aquatic animals in natural state, marking up for many of breeding shortcomings in laboratory. Biotelemetry is increasingly becoming one of most important research methods for investing the behavior and biology of aquatic animals. A completed acoustic tagging and tracking system was constructed to evaluate the application effect of this system and explore the behavior patterns of Channa argus and Carassius auratus in shallow macrophytic lakes. The VEMCO positioning system was used to tag and track the fish in the Shihoudian Lake located in Baiyangdian Lake, Hebei Province, from July 2019 to December 2019. The receivers of VR2Tx were used and the tracking tags were conducted with V8-4L. After the test of receivers’ performance in the Shihoudian Lake, the distance between the receivers was 60 m, when the reception rate was as high as 93%. MS-222 was used as anesthetic. The tags were implanted into the abdominal cavity of the fish body by surgical suture (the weight of tag and fish met the 2% rule). The postoperative results of Channa argus and Carassius auratus were compared. Studies showed that the mortality and infection rate of Carassius auratus were 75%, while those of Channa argus were only 41.67% and 13.33% respectively. After the tag was implanted, the narrow abdominal cavity of Channa argus should be eased and pressed to ensure that the probe did not compress the intestines to affect feeding and excretion. The receptive data showed that the receiver acquired more receptive data in the daytime than at night (P<0.05). The ANOVA results showed significant differences between the receptive data of sunny days and light rain (P<0.05). The Kendall and Spearman rank correlation test showed that the correlation between temperature and the receptive data was low. The results of path tracking indicated that Channa argus preferred the marginal area with high coverage of aquatic vegetation, intuitively demonstrating the habitat selectivity of Channa argus in its natural state. In this study, the applicability of biotelemetry in the shallow macrophytic lake was successful by constructing complete tracking system. These results support the application of the complete tracking system in the behavior of freshwater fish, and provide theoretical basis of ecological restoration and evaluate the restoration effect.