Abstract: To find an effective, economical and sustainable way of macroalgae artificial transplantation, a study based on the adhesive properties of spore tested several food additives in April, 2020. These adhesive mixtures were mixed with S. horneri spore and applied on the reef both inside and outside the laboratory. After observing the attachment, germination and growth situation of spore, the optimal adhesive was picked out. Meanwhile, the study of low temperature preservation of macroalgae spore extended the cycle of macroalgae artificial transplantation. Our results showed that: (1) algin(C₆H₇O₆N_a)n was a better adhesive. Higher viscosity and higher volume guaranteed better protection against current and dilution, which helps to better attachment of spore. Adhesive mixture of S. horneri spore applied on the reef survived well in subtidal zone, with a survival density of 278 ind./m². However, only those sites with less exposure to wave and current had a good effect of transplantation in the intertidal zone. (2) Low temperature preservation of macroalgae spore prolonged the cycle of macroalgae artificial transplantation. Zygote of S. horneri preserved at (7±3)℃ still have adhesive ability after 23 days. This adhesion had a better result against current scour. (3) The spore mucus technology can be widely used in macroalgae spore artificial breeding and transplantation. This technology covered lab spore mucus uniform spray skill, water surface spore mucus spray transplantation skill, tidal zone spore mucus uniform spray skill, subtidal zone spore mucus daub skill, and artificial reefs or algal reefs spore mucus uniform spray transplantation skill. The research provided a new way to transplant macroalgae, and will contribute to the development of adhesive exploit, spray method and facilities.