Abstract: This study evaluated the effects of diets containing maggot meal (MGM) in substitution of fishmeal (FM) on growth performance and antioxidant capacity of hybrid yellow catfish. Six isonitrogenous (crude protein content was 42%) experimental diets were formulated by replacing 0 (G0 as control diet), 20% (G20), 40% (G40), 60% (G60), 80% (G80), and 100% (G100) of FM with MGM. Each diet was fed to triplicate groups of fish for 8 weeks with the initial fish weight of (0.62±0.01) g. Growth performance and antioxidant enzyme activity (SOD, CAT, GR, T-AOC) in liver tissue and serum were determined at the end of the trial. The results showed that: (1) Compared with the control diet, FM had no significant effect on growth performance in G20 and G40 (P>0.05), and significant negative effect on G60, G80 and G100 group effect (P<0.05). Compared with the G0 diet, FM had no significant effect on feed conversion ratio (FCR) in G20 and G40 (P>0.05) and a significant negative effect on G60, G80 and G100 group (P<0.05). FM had no significant effect on survival ratio. (2) Body composition and physical indexes between hybrid yellow catfish are similar among groups (P>0.05). (3) The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) in the serum and liver of hybrid yellow carp in the G20 group were slightly higher than those in the G0 group but without significant difference (P>0.05). Liver CAT activity and total antioxidant capacity (T-AOC) increased significantly in G40 (P<0.05) compared with control group. GR and SOD activities in liver, SOD activity and T-AOC in serum decreased significantly in the G80 group (P<0.05) compared with control group. In summary, the fly pupae protein can replace 40% of fish meal without significantly negatively affecting the growth performance, body composition and antioxidant capacity of hybrid yellow catfish.