Abstract: To establish an accurate, efficient and economical pedigree identification technique for the channel catfish and provide a scientific basis for the genetic evaluation of the channel catfish and family breeding, two 5-plex PCR reactions were established using 10 pairs of SSR markers with high polymorphism. 333 individuals from 13 full-sib families and 8 half-sib families were identified by the channel catfish pedigree identification system. The results showed that the average number of alleles at 10 loci was 9.8, the average observed heterozygosity was 0.9952, the average expected heterozygosity was 0.8092, and the average polymorphism information content was 0.7845. The cumulative exclusion probabilities of these three cases were 0.99996806, 0.99833267, and 0.99999998, respectively. The identification of the verified population was highly consistent with the pedigree with a true identification rate of 99.1%. The average LOD value between the offspring and the parents was between 13.30 and 24.70, with a confidence level of 95%. The number of alleles in the microsatellite locus selected in this study is abundant, and the polymorphism is high. Our pedigree identification system can quickly and accurately identify the family from the channel catfish breeding population.