Abstract: Cephalopods are marine economic animal resources with a short life cycle and rapid growth rates. However, there is still a blank in the research on its niche. In order to better find out the ecological changes and the rules of community succession in south coast of Zhejiang. We analyzed the utilization of habitat resources of cephalopods in the south of Zhejiang Province as well as their relationship, niche breadth and overlap of different species. This will have certain guiding significance for the formulation of marine resource management and protection policies and measures. A total of 43 survey stations were set in this area. The systematic surveys of 4 investigations were conducted in November 2015, February, May and August 2016. We analyzed the niche overlap and differentiation of gained cephalopod samples, using the index of relative importance, niche breadth, niche overlap and other ecological indicators coupled with nonmetric multidimensional scaling (nMDS) and canonical correspondence analysis (CCA). This research on the cephalopod niche will help to understand the utilization of habitat resources and the relationship among the cephalopods in this area. The results demonstrated that 14 species of cephalopod were captured in the surveyed area and dominant species were Loligo edulis, Abralia ultihamata, Sepiella inermis and Loligo beka, the niche of which ranged from 0.09 to 7.01. Loligo edulis, Abralia ultihamata, Euprymna berryi and Loligo beka were wide niche species; Euprymna morsei, Sepiella inermis and Loligo tagoi were medium niche species; the rest were narrow niche species. The niche overlap ranged between 0.00−0.70, varied greatly in inter-species. The main factor that affect cephalopod distribution was suspended matter, and then chlorophyll and salinity. Temperature and dissolved oxygen had relatively small effects on cephalopod distribution. These environmental factors comprehensively explained 83% variation of cephalopod population structure in this area.