Abstract: The golden shell line and black shell line are the colored selection strains for Crassostrea sikamea. The F₃ population were used to evaluate their growth, survival, gonadal development, and biochemical component (glycogen, triglyceride, total protein). The results suggested that the shell height of black shell line was significant higher than that of golden shell line at larvae and juvenile stages (9—40 days; P<0.05), while the golden shell line had higher growth in the shell height, shell length, total weight, total volume, and body weight compared to the black shell line, with a significant difference for the total weight (P<0.05). The golden shell line had a growth advantage over the black shell line at adult stage. The survival rate was higher in the golden shell line than that in the black shell line during the period of 15—450 days. The survival rates were 55.35% and 42.22% in the golden shell line at 360 days (June) and 450 days (October), respectively, which were 2.19 times and 3.49 times higher than that of the black shell line (25.27% and 12.12%, respectively). The sex ratio (female: male) was close to 1﹕1 both in the golden shell and black shell lines, and their gonadal development was synchronous, with the highest spawning during the period of June to September (360—450 days). Moreover, the golden shell line had a higher reproduction compared to the black shell line. The glycogen and triglyceride contents were higher in the adductor muscle, mantle, gill, and gonad of the golden shell line than those of the black shell line (P>0.05). Furthermore, the glycogen content gradually increased with the gametogenesis in the golden shell and black shell lines, in contrast, the triglyceride content gradually decreased in the same period. The total protein content was indifferent between two groups. The present study displays that there is a close relationship between the shell color and growth, survival, and biochemical content, which provides a basic reference for the synergistic selection of phenotypes and shell color in C. sikamea.