Abstract: The effect of clove oil on the live transportation of bream was studied in this paper. Based on a single factor experiment, the optimum conditions for preservation during transportation was determined by orthogonal experiments, and the changes in water quality indicators and their effects on fish biochemical indicators during live transportation were detected and analyzed. The results showed that the optimum conditions for preservation and transportation were an anesthetic solution concentration of 15 mg/L, a water temperature of 9°C and a fish-water ratio of 1﹕3. The survival time was extended by up to 50h, and the fish survival rate was 100%. In terms of the water body indexes, there were no significant changes in pH and dissolved oxygen. The ammonia nitrogen level in the anesthesia group increased from 0.049 mg/L to 4.034 mg/L within 48h. The number of microorganisms in the water increased significantly within 24h. The increase in ammonia nitrogen and the growth of microorganisms affected the survival of fish. In terms of the biochemical indexes of the fish body, the glycogen content in the muscles of the anesthesia group fish decreased significantly within 24h. The content of lactic acid increased significantly and then decreased, and the pH decreased first and then increased, indicating that glycogen consumption produced lactic acid during the transportation of live fish. Among the blood indexes of the fish body, AST activity, LDH activity, urea and creatinine (CREA) increased after 12h and increased significantly after 36h (P<0.05), and then they were decreased in the anaesthetic group. The results showed that the metabolic levels of liver and kidney tissue were affected by the prolongation of the survival time.