Abstract: This study investigated effects of different cyclical starvation-refeeding modes on survival, growth, and fatty acids and amino acids of Octopus minor with initial weight of (94.29±9.35) g and initial mantle length of (53.25±5.25) mm under the seawater temperature of 19.8—22.2℃ in four groups including control group (continuous feeding), S₁F₅ group (cyclic starvation 1d and refeeding 5d), S₂F₄ group (cyclic starvation 2d and refeeding 4d), S₃F₃ group (cyclic starvation 3d and refeeding 3d) and for 24d. The results revealed that growth rate, hepatosomatic index, weight gain ratio and final weight increased at first and then decreased with the increased starvation time, in which all the indicators of S₁F₅ group were significantly higher than those of the control group, but all indicators except the hepatosomatic index of S₃F₃ group were significantly lower than those of the control group. The survival rate of O. minor decreased with enhanced starvation time but without significant differences among all groups. The food ingestion enhanced with the increased starvation phase, and their values of all three experimental groups were significantly higher than the control group. The content of SFA, UFA, MUFA and PUFA in fatty acids was not significantly different with the control group with the highest values in S₁F₅. The values of TEAA, TAA and TEAA/TAA of O. minor were not significantly different from the control group. In conclusion, O. minor from S₁F₅ and S₂F₄ groups exhibited compensate growth and S₁F₅ group showed over-compensatory growth. Therefore, the feeding mode of cyclical 1d starvation and 5d re-feeding can be used in O. minor culture.