Abstract: To evaluate muscle nutritive values of the hybrid F₁ of female Culter alburnus × male Megalobrama terminalis (CA×MT), the proximate composition, amino acids levels, fatty acids profiles and mineral contents in muscle of Culter alburnus (CA), Megalobrama terminalis (MT), and CA×MT were measured. The results showed that muscle moisture of CA×MT were significantly lower than those of CA and MT (P<0.05), while the muscle crude protein of CA×MT was significantly higher than others (P<0.05). Muscle essential amino acids (EAA) of CA×MT was the highest one that was significantly higher than that of MT (P<0.05). According to amino acid score (AAS) and chemical score (CS), the first limiting amino acid was methionine+cysteine (Met+Cys) for all three kinds of fish. The percentage of unsaturated fatty acid (UFA) to total fatty acids of CA×MT in muscle was higher than those of CA and MT (P<0.05). The contents of iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) of three kinds of fish in muscle had no significant difference (P>0.05). In conclusion, the muscle of CA×MT has a higher nutritive value with higher protein contents, EAA and UFA.