Abstract: Growth rate, feeding, development of organs, disease resistance, adaptability, and self-regulation to the change of environment are all associated with fish health assessment. With the rapid development of aquaculture, the assessment of fish health condition is increasingly important. The present manuscript reviewed the evaluation procedures and indices related to fish health condition and available shortcomings, and also discussed the possibility of developing new methods to assess fish health condition using computer technology and biotechnology, which are expected to provide new insights for fish health assessment.