Abstract: This study was conducted to investigate the effect of transportation stress on the plasma biochemical indices, body color, fish quality of gibel carp Carassis gibelio var. CAS III, body weight of (116.46±2.09) g and water quality. Transportation was simulated on an automobile vibration bench for 4h. After 4h simulative transportation, the ammonia concentration of water in bags increased significantly (P<0.01), and the dissolved oxygen of water in bags was increased (P<0.05), while the pH value decreased significantly (P<0.05). Fish plasma cortisol, glucose and MDA concentration increased significantly (P<0.01), while plasma lactic acid concentration decreased (P<0.01). Plasma GSH-PX significantly decreased after 4h simulative transportation (P<0.05), and plasma SOD and CAT had a low value after simulative transportation (P>0.05). Dorsal skin showed significantly decreasing L^* values after 4h simulative transportation (P<0.01), and the abdomen and lateral line L^* values also decreased with no significance (P>0.05). While a^* (P>0.05) and b^* (P<0.05) values declined in these three parts after 4h transportation. Cohesiveness, chewiness and resilience decreased significantly in fillet (P<0.05). The fillet gumminess, springiness and adhesiveness were slightly decreased (P>0.05), while its hardness increased (P>0.05). The results indicated that transportation could induce stress response of gibel carp and reduce fish quality.