Abstract: To study the tolerance of Percocypris pingi retrodorslis to temperature, pH, and salinity, the juvenile Percocypris pingi retrodorslis were used as experimental objects. A single factor static acute toxicity test was used to study their tolerance to temperature, pH and salinity. The experimental results showed that their tolerance on temperature ranged from 0 to 32℃, and the lethal high and low temperature was 32℃ and 1℃, respectively. The optimum growth temperature was between 8 and 27℃. In addition, the optimum growth pH was 5.0—9.0. However, the juvenile fish began to die when pH was higher than 9.5 or lower than 4.7 (within 96h after treatment). The effects of different alkalinity on the survival of juvenile fish have significant difference (P<0.05), after the treatment for 24h, 48h, 72h and 96h, but there was no significant difference effects on the survival at different acidity level (P>0.05). TheLC₅₀ of acidity for the juvenile fish was 3.90, 3.96, 4.15 and 4.40 after treated for 24h, 48h, 72h and 96h, respectively. The LC₅₀ of alkalinity was 11.20, 11.10, 10.98 and 10.89 after the treatment of 24h, 48h, 72h and 96h, respectively. The juvenile fish began to die when salinity was higher than 7.5, and the effects of different salinity on the survival of juvenile fish were significantly different (P<0.05). TheLC₅₀ of salinity for juvenile fish was 10.30, 9.25, 9, 8.85 and 8.82 g/L at 12h, 24h, 48h, 72h and 96h after treatment, respectively, and two safe concentration (SC) levels were 0.89 g/L and 2.385 g/L. The Percocypris pingi retrodorslis has been listed as endangered species, and our data will provide a theoretical foundation for the artificial breeding and trans regional breeding of Percocypris pingi retrodorslis.