Abstract: This study investigated effects of different temperatures, exogenous hormones and parental weights of wild Mastacembelus armatus on artificial induced spawning and incubation. The results showed that the temperatures of 24—32℃ were fit to artificially spawn and incubate, and the best temperature was 28℃. The ovulation time was (39.43±2.87)—(58.43±1.25)h, and duration of incubation was (44.35±0.5)—(75.74±0.63)h. As the water temperature increased, the time of ovulation and incubation became shorter. The weight of the parents directly affected artificial induced spawning and incubation. Under induce of exogenous hormone oxytocin, the smaller parents turned mature faster than larger ones with shorter ovulation time. The three indexes of fecundity, fertilization rate and hatching rate were positively correlated with parental weight. Moreover, the bigger the parents were, the more and better eggs were. The combination of 3 exogenous hormones at proper dose can achieve oxytocin. The comprehensive analysis showed that two hormones mixed formula (DOM+LRH–A₂) produced poor results while the three conditions (HCG+DOM+LRH–A₂) at optimal dose of 1000 IU+5 mg+6 μg produced the best results.