Abstract: To study the association between GHRL gene and growth traits of Chinese Soft-shell Turtle (Pelodiscus sinensis), In this study, we detected and found 14 SNPs of GHRL gene of Pelodiscus Sinensis by DNA sequencing: C289T, G501T, T738C, G776T, A841G, T885C, T2960C, A2987T, G3390A, A3857C, G4718A, T4820C, A4850C and T4979C. The genotype and gene frequency of these SNPs in 120 Chinese Soft-shell Turtle were assayed by using the method of Bio-Mass Spectrometry. The result showed that all the loci were found in Hardy-Weinberg equilibrium in the Chinese Soft-shell Turtle population. The growth traits (body weight, body length, body width, body height and calipash length) of CT and CC diplotype were significantly higher than those of TT diplotype in locus C289T (P<0.05). The growth traits (Body weight, body length, body width and calipash length) of AB diplotype were significantly higher than those of AA diplotype in locus S2 (P<0.05). The growth traits (Body length and body width) of AG diplotype were significantly higher than those of AA diplotype in locus G3390A (P<0.05). The growth traits (Body length, body width and calipash length) of AG diplotype were significantly higher than those of AA diplotype in locus G4718A (P<0.05). The results suggested that those loci identified in our study were closely related to the growth performance of Chinese Soft-shell Turtle; therefore it could demonstrating great potentials to be used as a potential molecular markers for better breeding.