Abstract: In this study, the high-throughput next-generation sequencing technology was used to build Lanzhou catfish (Silurus lanzhouensis) mitochondrial genome sequence, and the complete sequence and characteristics for mitochond-rial genome has been analyzed. The main results were demonstrated in detail. The circular genome was 16523 bp in length with the typical mtDNA components, and the base composition showed a tendency of high A+T and low G+C. Two promoters (ATG, GTG) and three terminators (TAG, TAA and T or TA) were found in all 13 PCGs. Except tRNA-Ser^(AGN) gene showed the shortage of DHU, the other 21 genes of tRNA could be folded in clover shape. The 45 stem-loop structures and 4 structural domains were identified in secondary structure of 12S rRNA, and 54 stem-loop structures and 6 structural domains were identified in 16S rRNA. Three different structural domains of termination sequence region (TAS1, TAS2), central conservative region (CSB-F, CSB-E and CSB-D) and conservative region (CSB1, CSB2 and CSB3) were divided in the controlled region that remains key sequence label. The non-code region has a conserva-tive sequence to control light chain starting copy. The complete sequence of mitochondrial genome and general COX1 label could be used for the study of evolutionary relationship between S. lanzhouensis and other Siluriformes species.