Abstract: Allogynogenetic gibel carp is a joint name of a series of clone strains bred from the gibel carp (Carassius gibelio Bloch). The numbers and distribution of intermuscular bones (IBs) are valuable economic traits. In this study, we compared the differences in number, morphology and distribution of IBs in clone A⁺ and clone F of an allogynogenetic gibel carp. The average numbers of clone F IBs at six months and 18 months are 71.8±2.9 and 83.3±1.4, respectively; both numbers are significantly less than those of clone A⁺ (78.6±3.9 and 87.0±1.5) (P<0.01). Similarly, the average numbers of clone F IBs within each sarcomere at 6-month and 18-month are remarkably smaller than those of clone A⁺. Both clone A⁺ and F have epineuralia bones (ENBs) and hypomeralia bones, and no epicentralia bones. The average numbers of clone F ENBs in the left body side at 6-month and 18-month are 48.2±1.1 and 55.8±0.5, respectively; both numbers are significantly less than those of clone A⁺ (53.7±1.6 and 58.7±0.5) (P<0.01). There was no difference found in the epipleural bones (EPBs) of clone A⁺ and clone F. The IBs of these two clone strains are divided into seven shape categories, including non-forked (I), one-end-unequal-bi-fork (卜), one-end-equal-bi-fork (Y), one-end-multi-fork, two-end-bi-fork, two-end-multi-fork, and tree-branch types. Generally, both numbers and morphological complexity of ENBs are higher than those of EPBs. The numbers of total IBs and IBs with complicated shapes are positively related with age. In the trunk on axis, clone F has fewer " Y” type ENBs and more " I” type ENBs than those of clone A⁺, indica-ting that clone F exhibits an improved economic trait. Therefore, our results provide an evaluative criterion for a novel strain (clone F) and basic data for genetic improvement of IBs in gibel carp.