Abstract: An 8-week feeding trial was conducted in indoor culture system to evaluate effects of replacing fish meal with soybean meal (SBM) on growth performance, body composition and apparent digestibility coefficients of juvenile Pseudobagrus ussuriensis initial body weight of (0.500.00) g. Seven isonitrogenous diets groups were formulated to replace fish meal protein by SBM at 0 (S0), 10% (S10), 20% (S20), 30% (S30), 40% (S40), 50% (S50) and 60% (S60). To investigate the effect of supplementation with methionine, S60 added 0.3% methionine to formulated the 8th group (SM60). Weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) linearly decreased with increasing SBM replacement level from 0 to 60%. There were no significant differences in WGR, SGR and PER among groups of S0, S10, S20, S30, and S40 (P0.05), but S50 and S60 groups had lower WGR, SGR and PER than S0 group (P0.05). No significant differences were observed in growth between S60 group and SM60 (P0.05) group, but both of them had significantly lower growth than S0 group. Feed conversion ratio (FCR) had an opposite trend with WGR and SGR. S0 group had significantly lower muscle moisture content than other groups (P0.05). Whole body and muscle protein contents decreased with increasing dietary SBM levels, and S60 and SM60 groups had significantly lower protein content than other groups (P0.05). Whole body and muscle lipid contents increased with increasing dietary SBM levels, and S60 and SM60 groups had significantly higher lipid contents than other groups (P0.05). S60 and SM60 groups had higher VSI and HSI than other groups (P0.05). Apparent digestibility coefficients (ADC) of protein and dry matter of S50 and S60 groups were significantly lower than other groups (P0.05). The ADC of phosphorus significantly increased with increasing SBM levels in the diet (P0.05). These results suggest that SBM at not more than 40% of fish meal protein replacement have no significantly negative effect on growth or feed efficiency, but higher dietary SBM levels reduce fish performance.