不同形式蛋氨酸对建鲤生长性能及血清游离氨基酸含量的影响
EFFECTS OF DIFFERENT FORMS OF DIETARY METHIONINE ON THE GROWTH AND FREE AMINO ACIDS IN SERUM OF JIAN CARP
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摘要: 为考察不同形式蛋氨酸对建鲤生长的作用效果, 实验以豆粕、鱼粉、棉粕为蛋白源, 配制缺乏蛋氨酸的基础饲料(对照组, 蛋氨酸含量为0.48%), 在基础饲料中分别添加晶体蛋氨酸、微囊蛋氨酸、蛋氨酸羟基类似物(MHA)及蛋氨酸羟基类似物钙盐(MHA-Ca), 使蛋氨酸含量达到0.58%, 获得5个饲料处理组, 饲养平均体重为(8.61.0) g的建鲤(Cyprinus carpio var Jian)8周。结果显示: 各组鱼体增重率分别为343.51%、350.77%、382.80%、384.02%和385.59%; 饲料系数分别为1.58、1.55、1.42、1.42和1.41; 晶体蛋氨酸组鱼体增重率、饲料系数与对照组无显著差异(P0.05), 微囊蛋氨酸组、MHA组、MHA-Ca组增重率较对照组提高11.4%、11.8%、12.2% (P0.05), 饲料系数降低10.1%、10.1%、10.8% (P0.05)。各处理组在肌肉水分、脂肪含量间无显著差异(P0.05), MHA组肌肉粗蛋白含量较晶体蛋氨酸组显著下降, 其他各组间无显著差异(P0.05)。对摄食后不同时间的血清游离氨基酸浓度变化的分析表明, 对照组在摄食后2h或3h达到峰值, 晶体蛋氨酸组、MHA组在摄食后1h达到吸收峰值, 微囊蛋氨酸组在摄食后1h或2h达到峰值, 而MHA-Ca组则在摄食后3h达到峰值。上述结果表明, 在蛋氨酸缺乏的颗粒饲料中补充晶体蛋氨酸, 对建鲤生长性能无改善作用, 而添加微囊蛋氨酸、蛋氨酸羟基类似物、蛋氨酸羟基类似物钙盐则显著提高了鱼体生长性能, 降低饲料系数。Abstract: In the present study, a methionine-eficient (0.48% methionine) diet was formulated as control. Crystal methionine, encapsulated methionine, methionine hydroxy analogue (MHA), and methionine hydroxyl analog calcium salt (MHA-Ca) were added separately into the control diet to form four experimental diets with the methionine level at 0.58%. Jian carp with an initial body weight of 8.6 g were fed with these five diets and their growth rates were compared after eight weeks. The weight gain (WG) of the control group, crystal methionine group, encapsulated methionine group, MHA group and MHA-Ca group were 343.51%, 350.77%, 382.80%, 384.02%, 385.59% respectively; the feed conversion ratios (FCRs) of the five groups were 1.58, 1.55, 1.42, 1.42, 1.41 respectively. Compared to the control group, the WG increased by 11.4%, 11.8%, 12.2% in the encapsulated methionine group, the MHA group and the MHA-Ca group respectively (P0.05); the FCR decreased by 10.1%, 10.1%, 10.8% respectively (P0.05). However the growth was not affected by the addition of crystal methionine. There were no significant differences in the muscle moisture and crude fat contents between the five groups. The crude protein content in the muscles from the MHA group was significantly lower than that from the crystal methionine group. The free essential amino acids (FEAA) in the serum peaked at the first hour after feeding for both the crystal methionine group and the MHA group, which was one hour earlier than the control group. The serum FEAA of the encapsulated methionine group reached the maximum around the same time as the control group. The serum FEAA of the MHA-Ca group was observed to peak three hours after feeding. These results indicated that the growth of Jian carp could be improved by the addition of dietary encapsulated methionine, MHA, and MHA-Ca, but not crystal methionine.
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Keywords:
- Jian carp /
- Crystal Met /
- Encapsulated Met /
- MHA /
- Growth /
- Serum free amino acids
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