长江流域种群遗传多样性和遗传结构分析
THE PATTERN OF UPPER AND MIDDLE YANGTZE DRAINAGES SHAPES THE GENETIC STRUCTURE AND DIVERSITY OF HEMICULTER LEUCISCULUS REVEALED BY MITOCHONDRIAL DNA LOCUS
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摘要: 鱼类群体遗传学研究主要集中在经济鱼类或濒危物种，然而一些经济价值较低的物种的遗传结构却甚少关注。因此，研究选择了经济价值较低的（Hemiculter leucisculus），共计323尾个体分别来自13个长江流域及其附属湖泊的自然群体。通过扩增线粒体DNA Cytb基因序列片段（1100 bp），以探讨种群遗传结构和遗传多样性。遗传多样性分析呈现出高单倍型多样性和高核苷酸多样性的模式，表明该种群在长江流域较为稳定。另外，基于线粒体细胞色素b基因的系统发育分析，显示有5个线粒体谱系（谱系A-F）组成。中性检验和核苷酸错配分布分析均显示谱系A、B、E、F曾经历过种群扩张，并且呈现从上游向中游扩张的规律。谱系间较高且显著的遗传分化指数和显著的系统进化关系，均表明谱系A-F之间存在明显的遗传分化，暗示长江流域可能至少存在4个不同线粒体DNA水平上的种。种群的遗传结构和多样性可能受到了长江流域特定格局的影响。Abstract: Fish population genetics have mainly focused on economic fish or endangered species, while the knowledge on genetic structure of the fish with low economic value is limited. In the present study, we collected a total of 323 sharpbelly (Hemiculter leucisculus) individuals from 13 populations in Yangtze River Basin and the affiliated lakes to evaluate their genetic diversity and population structure by amplifying the mitochondrial DNA Cyt b gene fragments (1100 bp). Genetic diversity analysis showed the pattern of high haplotype diversity and high nucleotide diversity, suggesting that the populations in the Yangtze River Basin were fairly stable. In addition, based on mitochondrial cytochrome b gene phylogenetic analysis, there were five mitochondrial lineages (lineage A-F). The neutral test of Fu'Fs and mismatch distributions with negative Tajima's D values indicated that the lineage A, B, E, and F underwent population expansion, and the expansion was from the upper section to the middle section of the Yangtze drainage. Fst and phylogenetic analysis and the significant divergence among lineages A-F implied that there are at least four different species on the level of mitochondrial DNA in the Yangtze River Basin. The population genetic structure and diversity of Hemiculter leucisculus may reflect the distribution pattern in Yangtze River Basin.
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Keywords:
- Hemiculter leucisculus /
- Genetic structure /
- Cyt b /
- Genetic diversity
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[1] Chen Y Y. Fauna Sinica: Osteichthyes (Cypriniformes Ⅲ). 1st edition [M]. Beijing:Science Press. 1998, 163-171 [陈宜瑜. 中国动物志: 硬骨鱼纲. 第一版. 北京: 科学出版社. 1998, 163-171]
[2] Cao Y P, Wen Z G. The biology of Hemiculuter leucisculus in Baiyangdian Lake [J]. Journal of Hebei University, 1996, 16: 27-33 [曹玉萍, 温振刚. 白洋淀白鲦鱼的生物学研究. 河北大学学报, 1996, 16: 27-30]
[3] Zeng G Q, L Y P, Huang P P, et al. Analysis of flesh content and nutritional component in the muscle of Hemiculter leucisculus Basilewksy and Sinibrama macrops Gunther [J]. Journal of Wenzhou University, 2012, 33: 1-7 [曾国权, 吕耀平, 黄佩佩, 等. 条、大眼华鳊含肉率和肌肉营养成分分析 温州大学学报, 2012, 33: 1-7]
[4] Wright S. The genetic structure of population structure [J]. Annals of Eugenics, 1951, 323-354
[5] Barluenga M, Meyer A. Old fish in a young lake: stone loach (Pisces: Barbatula barbatula) populations in Lake Constance are genetically isolated by distance [J]. Molecular Ecology, 2005, 14: 1229-1239
[6] Avise J C, Walker D, Johns G C. Speciation durations and Pleistocene effects on vertebrate phylogeography [J]. Proceedings of the Royal Society B-Biological Sciences, 1998, 265: 1707-1712
[7] Zardoya R, Doadrio I. Molecular evidence on the evolutionary and biogeographical patterns of European cyprinids [J]. Journal of Molecular Evolution, 1999, 49: 227-237
[8] Avise J C, Hamrick J L. Conservation Genetics, Case Histories from Nature [M]. New York: Chapman and Hall. 1996, 160-189
[9] Vrijenhoek R C. Genetic Diversity and Fitness in Small Populations [J]. Conservation Genetics, 1994, 68: 37-53
[10] Yuan X P, Yan L, Xu S Y, et al. Genetic diversity of bronze gedgeon (Coreius heterodon) and largemouth bronze (C. guichenoti) in Yangtze River Basin [J]. Journal of Fishery Sciences of China, 2008, 15: 377-385 [袁希平, 严莉, 徐树英, 等. 长江流域铜鱼和圆口铜鱼的遗传多样性. 中国水产科学, 2008, 15: 377-385]
[11] Grant W S, Bowen B W. Shallow population histories in deep evolutionary lineages of marine fishes: Insights from sardines and anchovies and lessons for conservation [J]. Journal of Heredity, 1998, 89: 415-426
[12] Yu F Y. Study on genetic diversity of Siniperca chuatisi/kneri complex in the Yangtze River [D]. Jinan University Master's Degree Thesis. Guangzhou. 2011 [余帆洋. 长江鳜/大眼鳜复合种的遗传多样性研究. 暨南大学硕士学位论文. 广州. 2011]
[13] Zhou L. Studies on genetic diversity and Structure of mtDNA control region of Mystus macropterus Bleeker [D]. Southwest University Master's Degree Thesis Chongqing. 2008 [周丽. 大鳍鳠(Mystus macropterus Bleeker)遗传多样性及其线粒体控制区结构的研究. 西南大学硕士学位论文. 重庆, 2008]
[14] Wright S. Evolution and the Genetics of Populations: Variability Within and Among Natural Populations [M]. Chicago: University of Chicago Press. 1984, 590
[15] Tajima F. DNA polymorphism in a subdivided population: the expected number of segregating sites in the two- subpopulation model [J]. Genetics, 1989, 229-240
[16] Tang G Z, Tao M. Discussion on relationship between the middle pleistocene glaciation and formation of the Yangtze Gorges [J]. Geology and Mineral Resources of South China, 1997, 10-16 [唐贵智, 陶明. 论长江三峡形成与中更新世大姑冰期的关系. 华南地质与矿产, 1997, 10-16]
[17] Xiang F, Zhu L D, Wang C S, et al. Character of Basaltic Gravels in Quaternary Sediments in Yichang Area and Its Relationship with Formation of Yangtze Three Gorges [J]. Journal of Earth Sciences and Environment, 2006, 28: 6-11 [向芳, 朱利东, 王成善, 等. 宜昌地区第四纪沉积物中玄武岩砾石特征及其与长江三峡贯通的关系. 地球科学与环境科学报, 2006, 28: 6-11]
[18] Liu J L, Ni Y Y, Chu G Q. Main paleoclimatic events in the quaternary [J]. Quaternary Science, 2001, 21: 239-248 [刘嘉麟, 倪云燕, 储国强. 第四纪的主要气候事件. 第四纪研究, 2001, 21: 239-248]
[19] Wright S. The Interpretation of Population-Structure by F-Statistics with Special Regard to Systems of Mating [J]. Evolution, 1965, 19: 395-420
[20] Su R F, Yang J X, Cui G H. The nominal invalidity of the cyprinid genus, Parasinilabeo, with descriptions of a new genus and species [J]. Zoological Studies, 2001, 40: 134-140
[21] Perdices A, Cunha C, Coelho M M. Phylogenetic structure of Zacco platypus (Teleostei, Cyprinidae) populations on the upper and middle Chang-Jiang (= Yangtze) drainage inferred from cytochrome b sequences [J]. Molecular Phylogenetics and Evolution, 2004, 31: 192-203
[22] Perdices A, Cunha C, Coelho M M. Mitochondrial diversity of Opsariichthys bidens (Teleostei, Cyprinidae) in three Chinese drainages [J]. Molecular Phylogenetics and Evolution, 2005, 37: 920-927
[23] Yang L, Mayden R L, He S P. Population genetic structure and geographical differentiation of the Chinese catfish Hemibagrus macropterus (Siluriformes, Bagridae): Evidence for altered drainage patterns [J]. Molecular Phylogenetics and Evolution, 2009, 51: 405-411
-
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