Abstract: By using central composite design, the join effects of water temperature (1634℃), salinity (1545), and pH (6.09.0) on the growth of Chlorella sp. CHX-1 were examined in this study. The results showed that the linear and quadratic effects of temperature, salinity and pH on the specific growth rate (SGR) of Chlorella were significant (P0.01). The interactive effect of temperature and salinity, interactive effect of temperature and pH were significant (P0.05), but the interaction between salinity and pH was not significant (P0.05). The effect of temperature on the SGR was greater than that of pH, which in turn was greater than that of salinity. Through response surface methodology, a model equation about the relationship of the growth rate to the three factors was established, with the R2, Adj.R2 as highly as 0.9759 and 0.9542, suggesting that the fitting capability of the model was satisfactory. The Pred.R2 reached to 0.8367, demonstrating that the model could be practicably applied for prediction. Through the optimization of the reliable model, the SGR reached its maximum (0.69) when the 3-factor combination was 26.7℃/25.5/7.3, with the desirability value being 0.999. These experimental results could offer theoretical reference for the production of the Chlorella.