饲料中添加丝兰提取物对大菱鲆幼鱼生长和生理及水环境的影响
EFFECTS OF DIETARY YUCCA SCHIDIGERA EXTRACT ON THE GROWTH PERFORMANCE, BLOOD PHYSIOLOGICAL AND BIOCHEMICAL INDICES OF TURBOT (SCOPHTHALMUS MAXIMUS) AND WATER ENVIRONMENT
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摘要: 用丝兰提取物添加量为0.00% (D0)、0.05% (D1)、0.10% (D2)、0.20% (D3)和0.40% (D4)的实验饲料投喂大菱鲆幼鱼[(42.200.06) g] 60d, 研究其对大菱鲆幼鱼生长性能、体组成、血清免疫代谢指标及养殖水质指标的影响。结果显示, 在饲料中添加丝兰提取物对大菱鲆幼鱼增重率(WGR)、特定生长率(SGR)、饲料系数(FCR)、脏体比(VSI)、肝体比(HSI)、肥满度(CF)均无显著影响(P 0.05)。全鱼、肌肉及肝脏中水分、粗蛋白、粗脂肪含量变化无显著性差异(P 0.05)。D3组幼鱼血清溶菌酶显著高于其他各组(P 0.05), 而D4组各免疫指标明显低于对照组(P 0.05); D3和D4组幼鱼血清的谷丙转氨酶(ALT)、谷草转氨酶(AST)活力显著低于对照组。除A1-6h组外各实验组水体氨氮含量均显著低于对照组(P 0.05), 各实验组亚硝酸盐含量均显著高于对照组(P 0.05), 各实验组总氮、总磷、磷酸盐含量均与对照组无显著性差异(P 0.05)。研究证明, 在饲料中适量添加丝兰提取物能够显著增强大菱鲆幼鱼非特异性免疫能力, 并显著降低养殖水体氨氮含量, 对大菱鲆生长及体组成无显著影响。以非特异性免疫及水体氨氮含量为综合评定指标, 大菱鲆幼鱼饲料中丝兰提取物的适宜添加量为0.20%。Abstract: In order to assess the effect of Yucca extract on growth performance, nutritional composition, serum immune of juveniles and aquaculture water environment, five formula feeds contained Yucca Extract at levels of 0.00% (D0), 0.05% (D1), 0.10% (D2), 0.20% (D3) and 0.40% (D4) were fed with juvenile Turbot (Scophthalmus maximus). The result showed that, no significant difference occurred in weight gain, specific growth rate, feed conversion ratio, viscerosomatic index, hepatosomatic index and condition factor of fish among all dietary treatments (P 0.05). Water, crude protein and crude lipid content of whole fish, muscle and liver exhibited no significant difference among groups (P 0.05). Serum lysozyme was enhanced in fish fed diet D3 significantly, while all immune indices were reduced in fish fed diet D4. The activities of alanine transaminase and aspartate aminotransferase of fish fed diet D3 and D4 were lower than that of the control dietary treatment (P 0.05). Except D1-6h, Ammonia content of all dietary treatments were significantly lower than that of the control treatment (P 0.05). Nitrite content of all dietary treatments were higher than that of the control treatment (P 0.05), while total nitrogen, total phosphorus and phosphate content showed no significant difference to the control treatment (P 0.05). The optimum content of Yucca Extract for juvenile turbot was 0.20% of weight based on the consideration of non-specific immunity and ammonia nitrogen content in aquaculture water.
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[1] Lu B, Wa E G. Application of Yucca schkdigera extract in shrimp feed [J]. Guangdong Feed, 2002, 11(1): 3234 [鲁滨, 瓦尔格. 阿丝兰提取物添加剂在虾饲料中的应用. 广东饲料, 2002, 11(1): 3234]
[2] Santacruz-Reyes R A, Chien Y H. Efficacy of Yucca schidigera extract for ammonia reduction in freshwater: Effectiveness analysis and empirical modeling approach [J]. Aquaculture, 2009, 297: 106111
[3] Gaber M M. The effects of plant-protein-based diets supplemented with Yucca on growth, digestibility, and chemical composition of Nile Tilapia (Oreochromis niloticus, L) fingerlings [J]. Journal of the World Aquaculture Society, 2006, 37(1): 7481
[4] Su Z F, Zhang H B, Fan Y Y. Effects of yucca extracts and Bacillus subtilis feed additive on broiler growth performance and odour controIin broiler house [J]. Feed Review, 2012, 1: 16 [苏子峰, 张红兵, 樊月圆. 丝兰提取物与枯草芽孢杆菌对肉鸡生长性能及鸡舍有害气体的影响. 饲料博览, 2012, 1: 16]
[5] Liang G Q, Wang X P, Wang X M. Effects of camphor familial plant extract and yucca extracts on emission of NH3 and H2S in slurry of weaned pigs [J]. Chinese Journal of Animal Science, 2009, 45(13): 2226 [梁国旗, 王旭平, 王现盟. 樟科、丝兰属植物提取物对仔猪排泄物中氨和硫化氢散发的影响. 中国畜牧杂志, 2009, 45(13): 2226]
[6] Cheng Z B, Fan Y Y, Zhang H B. Effects of Yucca extracts and Bacillus subtilis feed additive on utilization of broiler feed protein [J]. Feed Research, 2012, 12: 13 [程志斌, 樊月圆, 张红兵. 丝兰提取物与枯草芽孢杆菌对肉鸡饲料蛋白利用的影响. 饲料研究, 2012, 12: 13]
[7] Tidwell J H, Carl D, Webster Julia A, et al. Effects of Yucca shidigera extract on water quality and fish growth in recirculating-water aquaculture systems [J]. The Progressive Fish-Culturist, 1992, 54(3): 196201
[8] Ho P C, Chien Y H. Effect of Yucca schidigera extract addition on ammonia concentration in water for live transport of milkfish (Chanos chanos) juvenile. Book of Abstracts. Fisheries Society of Taiwan, Annual Conference, 59
[9] Santacruz-Reyes R A, Chien Y H. Ammonia reduction in seawater by Yucca schidigera extract: efficacy analysis and empirical modeling [J]. Aquaculture Research, 2010a, 41: 12211228
[10] Santacruz-Reyes R A, Chien Y H. Yucca schidigera extract-A bioresource for the reduction of ammonia from mariculture [J]. Bioresource Technology, 2010b, 101: 56525657
[11] Santacruz-Reyes R A, Chien Y H. The potential of Yucca schidigera extract to reduce the ammonia pollution from shrimp farming [J]. Bioresource Technology, 2012, 13: 311314
[12] El-Saidy, Gaber M M A. Use of cottonseed meal supplemented with iron for detoxication of gossypol as a total replacement of fishmeal in Nile tilapia, Oreochromis niloticus (L.) diets [J]. Aquaculture Research, 2004, 34(13): 11191127
[13] Gaber M M. The effects of plant-protein-based diets supplemented with Yucca on growth, digestibility, and chemical composition of Nile Tilapia (Oreochromis niloticus, L) fingerlings [J]. Journal of the World Aquaculture Society, 2006, 37(1): 7481
[14] Lei Q L. Overview of turbot farming in Britain [J]. Fisheries Science Technology Information, 1983, 2: 2627 [雷霁霖. 英国大菱鲆养殖概况. 水产科技情报, 1983, 2: 2627]
[15] Nie Q, Miao H J, Miao S Y, et al. Effects of dietary carbohydrate sources and levels on the activities of carbohydrate metabolic enzymes in turbot [J]. Acta Hydrobiologica Sinica, 2013, 37(3): 425433 [聂琴, 苗惠君, 苗淑彦, 等. 不同糖源及糖水平对大菱鲆糖代谢酶活性的影响. 水生生物学报, 2013, 37(3): 425433]
[16] Lei Q L. Turbot Farming Techniques [M]. Shanghai: Shanghai science and Technology Press. 2003, 8 [雷霁霖. 大菱鲆养殖技术. 上海: 上海科学技术出版社. 2003. 8]
[17] Ma A J, Lei Q L, Chen S Q, et al. Genetic parameterization for Turbot Scophthalmus maximus: implication to breeding strategy [J]. Oceanologia et Limnologia Sinica, 2002, 33(1): 7582 [马爱军, 雷霁霖, 陈四清, 等. 大菱鲆不同产卵季节对卵子的生物学及生化特征的影响. 海洋与湖沼, 2002, 33(1): 7582]
[18] Zhou J, Song X L, Huang J, et al. Effects of dietary supplementation of A3 alpha-Peptidoglycan on innate immune responses and defense activity of Japanese fiounder (Paralichthys olivaceus) [J]. Aquaculture, 2006, 251(2-4): 172181
[19] Zhou J, Song X L, Huang J, et al. The optimal oral strategy of A3-peptidoglycan on Japanese flounder, Paralichthys olivaceus [J]. Marine Fisheries Research, 2005, 26(4): 1925 [周进, 宋晓玲, 黄捷, 等. 牙鲆口服A3a一肽聚糖最佳投喂方案的选择. 海洋水产研究, 2005, 26(4): 1925]
[20] Wang Y J. Effect and mechanism of several non-nutritional and non-pollution additives on Turbot (Scophthalmus maximus L.) [D]. Thesis for Master of Science. Institute of Oceanology, Chinese Academy of Sciences, Qingdao. 2006 [王优军. 几种非营养无公害添加剂对大菱鲆的效应和机理研究. 硕士学位论文, 中国科学院海洋研究所, 青岛. 2006]
[21] Santarem M, Novoa B, Figueras A. Effects of beta-glucans on the non-specific immune responses of turbot (Scophthalmus maximus L.) [J]. Fish Shellfish Immunology, 1997, 7(6): 429443
[22] Aspila K L, Agemian H, Chau A S Y. A seni-automated method for the determination of inorganic and total phosphate in sediments [J]. Analyst, 1976, 101: 187197
[23] Xu H Y. Effects of dietary cactus extracts on growth performance, protein efficiency, oxygen consumption and ammonia excretion of Trachinotus blochii fry [D]. Thesis for Master of Science. Taiwan Ocean University, Taiwan, China. 2008 [许纮瑜. 饲料添加仙人掌萃取物对黄腊鲳鱼苗成长、蛋白质效率、耗氧与排氨之影响. 硕士学位论文, 中国台湾海洋大学, 台湾. 2008]
[24] Roberto Adrin Santacruz-Reyes. The application of Yucca schidigera extract in shrimp culture [D]. Thesis for Master of Science. Taiwan Ocean University, Taiwan China. 2010 [尚瑞柏. 丝兰萃取物在虾养殖的应用. 硕士学位论文, 台湾海洋大学, 中国台湾. 2010]
[25] Kelly A M, Kohle C C. Effects of Yucca shidigera extract on growth, nitrogen retention, ammonia excretion, and toxicity in channel catfish Ictalurus punctatus and hybrid tilapia Oreochromis rnossambicusO. niloticus [J]. Journal of the World Aquaculture Society, 2003, 34(2): 156161
[26] Huang K K, Zhang C X, Wang L, et al. Effects of dietary grobiotic-A and Yucca schidigera extract on growth performance in bullfrog Rana catesbeiana [J]. Fisheries Science, 2013, 32(5): 267273 [黄康康, 张春晓, 王玲, 等. 乳源性益生元和丝兰提取物对牛蛙生长的影响. 水产科学, 2013, 32(5): 267273]
[27] Ding Y M, Yang Z B, Yang W R, et al. Effects of different level biopowder on growth performance, and biochemical prarmeters of blood in broilers [J]. Journal of Shangdong Agricultural University (Natural Science). 2011, 42(1): 7074 [丁永敏, 杨在宾, 杨维仁, 等. 不同水平丝兰提取物对肉鸡生产性能和血液生化指标影响的研究. 山东农业大学学报(自然科学版), 2011, 42(1): 7074]
[28] Kaneda N H, Nakanishi, E J Staba. Steroidal constituents of Yucca shidigera plants and tissue cultures [J]. Phytochemistry, 1987, 26: 14251429
[29] Yang Z G, Xin C, Zheng J W. Application test of Panax Notoginseng Saponins in the feed for Tilapia nilotica [J]. Feed Research, 2008, (10): 5152 [杨志刚, 忻晨, 郑剑伟. 三七总皂苷在罗非鱼饲料中的应用试验. 饲料研究, 2008, (10): 5152]
[30] Chen A Q, Liu Z W, Huang Q. Effect of Panax Notoginseng Saponins on growth performance and body composition of gibel carp (Carassius auratus gibelio) [J]. Feed Research, 2011, 1: 6365 [陈阿琴, 刘志伟, 黄庆. 三七总皂苷对异育银鲫生长及体成分的影响. 饲料研究, 2011, 1: 6365]
[31] Xiang X, Chen J, Zhou X H, et al. Effect of astragalus polysaccharides on growth, body composition and immune index in schizothorax prenanti [J]. Acta Hydrobiologica Sinica, 2011, 35(2): 291299 [向枭, 陈建, 周兴华, 等. 黄芪多糖对齐口裂腹鱼生长、体组成和免疫指标的影响. 水生生物学报, 2011, 35(2): 291299]
[32] Wang M C, Yu R P, Xiao F Q, et al. Effects of Yucca extracts on the morphology of broiler mucosa [J]. Feed Industry, 2007, 28(11): 4344 [王勉超, 余锐萍, 肖发沂, 等. 丝兰属植物提取物对肉鸡肠黏膜形态结构的影响. 饲料工业, 2007, 28(11): 4344]
[33] Tang J, Zhu Y W, Hou S S, et al. Effect of feeding diets containing Yucca schidigera extract and Quillaja saponaria extract on growth performance and intestinal villus morphology of Beijing ducks [J]. Chinese Journal of Animal Science, 2012, 7: 5154 [唐静, 朱勇文, 侯水生, 等. 丝兰属麟凤兰和皂皮树提取物对北京鸭生产性能、小肠绒毛形态的影响. 中国畜牧杂志, 2012, 7: 5154]
[34] Koshikawa N, Hasegawa S, Nagashima Y, et al. Expression of trypsin by epithelial cells of various tissues, leukocytes, and neurons in human and mouse [J]. American Journal of Pathology, 1998, 153(3): 937944
[35] Li G X, Zhu X H, Wang M Z, et al. Effect of Yucca schidigera Extract on rumen microbial composition and methane production [J]. China Feed, 2008, 7: 3336 [李国祥, 朱小惠, 王梦芝, 等. 丝兰提取物对瘤胃微生物组成及甲烷生成的影响. 中国饲料, 2008, 7: 3336]
[36] Callewaert L, Michels C W. Lysozymes in the animal kingdom [J]. Journal of Biosciences, 2010, 35(1): 127160
[37] Van Herreweghe J M, Vanderkelen L, Callewaert L. Lysozymes inhibitor conferring bacterial tolerance to invertebrate type lysozyme [J]. Cellular and Molecular Life Sciences, 2010, 67: 11771188
[38] Carmelo N J, Bobbi L H. A unique perspective biomed pharmacother [J]. Arginine and Immunity, 2002, 56(10): 471482
[39] Fridovich I. Superoxide dismutases: An adaptation to a parmagnetic gas [J]. Journal of Biological Chemistry, 1989, 264: 77617764
[40] Zhou J, Song X L, Huang J, et al. Effect of A3 peptidoglycan (PG) on SOD, ACP, and AKP activities in different tissues of Japanese flounder Paralichthys olivaceus [J]. Journal of Fishery Sciences of China, 2004, 11(4): 296301 [周进, 宋晓玲, 黄健, 等. A3肽聚糖对牙鲆不同组织中超氧化物歧化酶及磷酸酶活性的影响. 中国水产科学, 2004, 11(4): 296301]
[41] Zang Y Q, Tian X L, Dong S L, et al. Growth, metabolism and immune responses to evisceration and regeneration of viscera in sea cucumber, Apostichopus japonicus [J]. Aquaculture, 2012, (358359): 5060
[42] Maita M, Shiomitsu K, IkedaY. Health assessment by the climogram of hemochemical constituents in cultured yellow-tail [J]. Bulletin of the Japanese Society for the Science of Fish, 1984, 51: 205211
[43] Yang Q H, Tan B P, Dong X H, et al. Effect of diets with different levels of BioAqua on growth, non-specific immunity and water quality in Litopenaeus vannamei [C]. China Society of Fisheries Abstracts of the Annual Meeting 2010. 2011 [杨奇慧, 谭北平, 董晓慧, 等. 饲料中不同水平丝兰宝对凡纳滨对虾生长、非特异免疫指标及水质参数的影响. 2010年中国水产学会学术年会论文摘要集. 2011]
[44] Selcuk Z, Tuncer S D. The effects of different levels of yucca schidigera added to the lambs diets containing urea on growth performance, carcass characteristics, some rumen and blood parameters [J]. Journal of Animal and Veterinary Advances, 2010, 9(4): 654660
[45] Boyd C E, Tucker C S. Pond Aquaculture Water Quality Management [M]. Kluwer Academic Publishers, Boston, MA, USA. 1998, 700
[46] Chiayvareesajja S, Boyd C E. Effects of zeolite, formalin, bacterial augmentation, and aeration on total ammonia nitrogen concentrations [J]. Aquaculture, 1993, 116: 3345
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