Abstract: Effects of salinity on the growth, the non-specific immunity and the antioxidant enzyme activities in Obscure puffer Takifugu obscures were investigated. The salinity of aquaculture water were maintained at 6, 12, 18, 24 and 30 in four experimental groups, and group of fresh water was used as control. We collected the samples on day 1, 5, 10, 15, 20, 30, 40 and 50 during the experiment, and we examined the activities of lysozyme, alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) in the samples. The salinity did not show significant impact on the growth when the salinity of culture water was below 18. However, the growth was inhibited when treated with salinity of 24 and 30, and the death rate was also elevated with the increase in salinity. The activities of both lysozyme and AKP was first enhanced by the increased salinity, but the activities in water with higher salinities (24 and 30) were significantly lower than that of in control groups. The activities of SOD and CAT also increased after treatment, then gradually decreased and the final level was not significantly different from control groups. The results showed that high salinity of 24 and 30 significantly affected the growth, non-specific immunity and the activities of antioxidant systems in obscure puffer Takifugu obscures.