神经坏死病毒在赤点石斑鱼组织中的分布

DISTRIBUTION OF NERVOUS NECROSIS VIRUS IN THE JUVENILE RED-SPOTTED GROUPERS (EPINEPHELUS AKAARA) BY IN SITU HYBRIDIZATION AND IMMUNOFLUEORESCENT ASSAYS

摘要: 研究采用地高辛原位杂交和免疫荧光检测技术分别检测病毒RNA2和衣壳蛋白在患病赤点石斑鱼Epinephelus akaara稚鱼中分布。地高辛检测结果表明病毒RNA2主要分布在脑、脊髓、视网膜和鳃上；免疫荧光检测结果和地高辛检测结果一致，表明病毒靶器官主要也是脑、脊髓、视网膜和鳃。肠道中几乎检测不到阳性信号，可能不是病毒的靶器官。因此可以推测神经坏死病毒感染赤点石斑鱼的主要途径是鳃，而不是肠道。

Abstract: Nervous necrosis virus (NNV) is a pathogen that infects more than 30 species of economically important fish worldwide. It causes high mortality among juveniles, larvae and even adult fish, and results in significant threat to aq-uaculture industry. Studying the distribution of NNV in the infected fish may help to understand the pathogenicity. In the present study, we used DIG-in situ hybridization and immunofluorescent assays to evaluate NNV distribution in the infected red-spotted grouper juveniles. The results of DIG-in situ hybridization showed that viral RNA2 was mainly detected in the brain, spinal cord, retina and gill. The immunofluorescent staining results indicated that viral capsid protein was mainly observed in the same tissues. However, both RNA2 and capsid protein were barely detected in the intestine, which indicated that the virus infection may go through the gills instead of the intestine.