Abstract: Trionyx sinensis is one of precious and high economic valued aquatic animals, which has important edible and medical value. Trionyx sinensis has been intensively artificially fed in our country. However, the outbreak of diseases happened frequently in Trionyx sinensis culture, due to aquaculture water pollution caused by the baits and feces left in culture. The aquaculture wastewater in Trionyx sinensis culture was discharged at will, which also lead to serious environmental pollution. In order to solve the environmental pollution problem produced by Trionyx sinensis culture, the complex photosynthetic bacteria and Bacillus subtillis were immobilized by sodium alginate and zeolite. The wastewater from Trionyx sinensis culture pond was treated with immobilized complex photosynthetic bacteria, immobilized Bacillus subtillis, Ipomoea aquatica, alone or in combination. The effects of wastewater purification were investigated. Results showed that the treatment performance was the best in the four different methods, when the wastewater was purified by Ipomoea aquatica in combination with immobilized bacteria. The removal rates of CODMn, , NH4+-N,NO3--N,NO2--N, PO43- in wastewater were 88.7%, 87.3%, 90.8%, 98.3% and 74.9% respectively, after 15 days treating. The removal rate of NH4+-N , NO3--N and NO2--N treated by Ipomoea aquatica was much higher than those of immobilized complex photosynthetic bacteria and B. subtillis. The removal rates of NH4+-N,NO3--N and NO2--N treated by immobilized complex photosynthetic bacteria was much higher than that of immobilized B. subtillis. However, the removal rate of CODMn treated by immobilized B. subtillis was the highest, which was up to 90.6%. The results could be applied in aquaculture wastewater purification for reference.