Abstract: Digestibility is an important index for nutrition estimation of fish feed, but different faeces collection methods have been reported to result in great difference of apparent digestibility coefficient (ADC) estimation. This experiment investigated the faecal production fastigium and duration for gibel carp (Carassius auratus gibelio) (about 100 g); and compared the difference among the ADC values from different faeces collection methods. Chromic oxide (Cr2O3) at 1% was added as a marker. We chose three different faeces collection methods: faeces collector, siphoning from the tank bottom and intestine dissection; and set different faeces collection times in faeces collector and siphoning methods. The results revealed that, faecal production fastigium of normally feeding gibel carp was 5―10h after feeding; faecal production fastigium of pre-fasted gibel carp was 7―9h after feeding; ADC of dry matter (ADCd) and ADC of protein (ADCp) of gibel carp was low in initial phase of defecation, and then increased with the appearance of faecal production fastigium. Different faeces collection methods and times has significantly affected the determination of ADCd and ADCp for gibel carp (P<0.05). The ADCd for siphoning was similar to that for faeces collector at faecal production fastigium; the ADCd for dissection was lower than that of siphoning and faeces collector at faecal production fastigium. The ADCp for siphoning was lower than that of faeces collector at faecal production fastigium; the ADCd for dissection was lower than that of siphoning and faeces collector at all sampling times. On base of selecting fresh and complete faeces, siphoning was a convenient and credible faeces collection method for ADC determination. But dissection may induce more deviation facility, because of procedural errors.