Abstract:
Growth hormone is one of the key regulators in animal growth, and combines with growth hormone receptorsof membrane protein to function, so the mutations on growth hormone receptor gene have an important influence onanimal growth. We identified 38 SNPs on four jlGHRs, by sequencing the genes from 6 individuals of Cyprinus carpiovar. Jian. PCR-RFLP was applied to detect the genotypes of 5 SNPs loci (1a intron 3 A43G, 1a exon 8 A361G, 1b exon8C12T, 2a exon 8 A555G and 2b exon 8 A315G) among 353 individuals and the relationship between the differentgenotypes and growth characteristics was also analyzed in this study. Results showed that all the five sites were associatedwith weight gain significantly. SNP locus 1a intron 3 A43G has a significant correlation with height/length andthickness/length as well, 1b exon 8 C12T has a significant correlation with height/length and caudal peduncleheight/length, 1a exon 8 A361G and 2a exon 8 A555G also have a significant correlation with caudal peduncledepth/caudal length. Data analysis also showed that the individuals with more than four markers grew faster obviouslythan those with few markers. The number of the individuals with 2 markers is the most, with 30 percent in the investigatedpopulation. But the number of the individuals with more than four markers only has a percentage of 14, indicatingthe presence of large selection space. The 5 SNPs detected in this experiment can be used in molecular breeding.