刀鲚和凤鲚的分子鉴定及其在仔鱼种类鉴定中的应用
MOLECULAR IDENTIFICATION OF COILIA ECTENES AND COILIA MYSTUS AND ITS APPLICATION ON LARVAL SPECIES
-
摘要: 使用mtDNA Cyt b基因序列作为分子标记,基于400 bp左右的序列分析长颌鲚、短颌鲚及凤鲚成鱼的遗传关系。结果显示长颌鲚和短颌鲚间的遗传距离在0—0.5%之间;凤鲚与长颌鲚、短颌鲚的遗传距离分别在7.9%—8.9%和7.7%—8.6%之间。用Kimura双参数模型构建的NJ分子系统树显示,长颌鲚和短颌鲚不能形成彼此独立的分支,而是混合聚在一起形成一个分支;凤鲚则形成另一个分支。对长江口沿岸区域的鲚属仔鱼Cyt b基因片段序列分析发现,仔鱼个体之间的遗传距离为0—0.7%,仔鱼与长颌鲚、短颌鲚的遗传距离在0.1%—0.5%之间,而与凤鲚的遗传距离为8.3%—8.8%。在Kimura双参数模型构建的NJ分子系统树中,仔鱼与长颌鲚、短颌鲚聚为一类,而与凤鲚分开,表明分析的仔鱼样品不包括凤鲚个体。由于长江口一般没有短颌鲚分布,这些仔鱼应该都是长颌鲚。Abstract: Based on about 400 bp sequences, nucleotide sequences of Cytochrome (Cyt b) segments of mtDNA wereanalyzed for anadromous Coilia ectenes, freshwater resident C. ectenes and C. mystus in the Yangtze River, China, todevelop a reference data for larval species identification of genus Coilia. The genetic distance ranged from 0 to 0.5%between the anadromous and freshwater resident C. ectenes, 7.9% to 8.9% between C. mystus and the anadromous C.ectenes, and 7.7% to 8.6% between C. mystus and the freshwater resident C. ectenes. Neighbor-joining (NJ) moleculardendrogram using Kimura 2-parameter based on the sequences of Cyt b gene showed individuals of the anadromous andfreshwater resident C. ectenes mixed into a cluster, and those of C. mystus forming another cluster. Thus, larvae of C.mystus could be distinguished from that of C. ectenes through Cyt b analysis. Nucleotide sequences of Cyt b of larvae ofgenus Coilia collected from the Yangtze Estuary were analyzed, and compared to the reference established above. Geneticdistance ranged from 0.1% to 0.5% between the larvae and the anadromous and freshwater resident C. ectenes, and8.3% to 8.8% between the larvae and C. mystus. NJ molecular dendrogram showed that all the larvae and the anadromousand freshwater resident C. ectenes clustered together, while individuals of C. mystus forming a separate cluster.Thus, the larvae were not of C. mystus. As there is no freshwater resident C. ectenes inhabiting in the Estuary, the larvaeshould be of the anadromous C. ectenes.
-
Keywords:
- Yangtze Estuary /
- Coilia ectenes /
- Coilia mystus /
- Larvae /
- Species identification /
- Cyt b gene
-
-
[1] Musiy Y I, Sergiyenko V A. Finerlings of the genus Lutjanus(Lutjanidae, Perciformes) from the Gulf of Aden [J]. Journalof Ichthyology, 1977, 17: 151—154
[2] Leis J M, Carson-Ewart B M. The larvae of Indo-Pacificcoastal fishes [M]. 2nd edition, Leiden; Boston: Brill. 2004
[3] Yang G, Cai Y, Liu H. Identification of several imported andexported animal products by mitochondrial DNA [J]. ChineseJournal of Zoology, 2004, 39(5): 40—43 [杨光, 蔡垚,刘海. mtDNA 标记在几种海关进出口动物产品鉴定中的应用. 动物学杂志, 2004, 39(5): 40—43]
[4] Liu Z H, Kong J, Liu P, et al. Application of RAPD technologyfor identification in three different stocks of Penaeuschinensis [J]. Acta Oceanological Sinica, 2003, 23(4): 67—71 [刘振辉, 孔杰, 刘萍, 等. RAPD 技术在中国对虾群体鉴别上的应用. 海洋学报, 2003, 23(4): 67—71]
[5] Xiao W H, Zhang Y P. Genetics and evolution of mitochondrialDNA in fish [J]. Acta Hydrobiologica Sinica, 2000,24(4): 384—391 [肖武汉, 张亚平. 鱼类线粒体DNA 的遗传与进化. 水生生物学报, 2000, 24(4): 384—391]
[6] Zardoya R, Meyer A. Phylogenetic Performance of mitochondrialprotein-coding genes in resolving relationshipsamong vertebrates [J]. Molecular Biology and Evolution,1996, 3(7): 933—942
[7] Shi W G, Wang B. Status quo of tapertail anchovy resourcein the estuaries of the Yangtze River [J]. Acta HydrobiologicaSinica, 2002, 26(6): 648—653 [施炜刚, 王博. 长江河口区凤鲚的资源现状. 水生生物学报, 2002, 26(6):648—653]
[8] Liu K, Zhang M Y, Xu D P, et al. Studies on resourcechange and MSY of Coilia mystus in the Yangtze River estuary[J]. Journal of Shanghai Fisheries University, 2004,13(4): 298—303 [刘凯, 张敏莹, 徐东坡, 等. 长江口凤鲚资源变动及最大持续产量研究. 上海水产大学学报, 2004,13(4): 298—303]
[9] Yuan C M, Qin A L. Ecological habits and distribution ofCoilia along the Chinese coast and its changes of output [J].Marine Science, 1984, 5: 35—37 [袁传宓, 秦安舲. 我国近海鲚鱼生态习性及其产量变动状况. 海洋科学, 1984, 5:35—37]
[10] Zhang M Y, Xu D P, Liu K, et al. Studies on biological characteristicsand changes of resource of Coilia ectenes in thelower reaches of Yangtze River [J]. Resources and Environmentin the Yangtze Basin, 2005, 14(6): 694—698 [张敏莹,徐东坡, 刘凯, 等. 长江下游刀鲚生物学及最大持续产量研究. 长江流域资源与环境, 2005, 14(6): 694—698]
[11] Zhuang P, Wang Y H, Li S F, et al. Fishes of the YangtzeEstuary [M]. Shanghai: Shanghai Scientific & TechnicalPress. 2006, 157—160 [庄平, 王幼槐, 李圣法, 等. 长江口鱼类. 上海: 上海科学技术出版社. 2006, 157—160]
[12] Li Y, Xie S, Li Z, et al. Gonad development of an anadromousfish Coilia ectenes (Engraulidae) in lower reach ofYangtze River, China [J]. Fisheries Science, 2007, 73: 1224—1230
[13] Cheng Q T, Zheng B S. Systematic synopsis of Chinesefishes (I) [M]. Beijing: Science Press. 1987, 61 [成庆泰, 郑葆珊. 中国鱼类系统检索(上册). 北京: 科学出版社. 1987,61]
[14] Zhang S Y. Fauna Sinica (Osteichthyes: Acipenseriformes,Elopiformes, Clupeiformes, Gonorhynchiformes) [M]. Beijing:Science Press. 2001, 148—156 [张世义. 中国动物志.硬骨鱼纲, 鲟形目, 海鲢目, 鲱形目, 鼠目. 北京: 科学出版社. 2001, 148—156]
[15] Yuan C M, Lin J B, Qin A L. On the classification and statusquo of genus Coilia in China [J]. Journal of Nanjing University,1976, 2: 1—12 [袁传宓, 林金榜, 秦安舲. 关于我国鲚属鱼类分类的历史和现状. 南京大学学报, 1976, 2: 1—12]
[16] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: aLaboratory Manual (2nd edn) [M]. New York: Cold SpringHarbor Laboratory Press. 1989
[17] Xiao W H, Zhang Y P, Liu H. Molecular systematics ofXenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography,and coevolution of a special group restricted in EastAsia [J]. Molecular Phylogenetics Evolution, 2001, 18(2):163—173
[18] Thompson J D, Gibson T J, Plewniak F, et al. The Clustal Xwindows interface: Flexible strategies for multiple sequencesalignment aided by analysis tools [J]. Nucleic Acids Research,1997, 25(24): 4876—4882
[19] Tamura K, Dudley J, Nei M, et al. MEGA4: Molecular EvolutionaryGenetics Analysis (MEGA) software version 4.0 [J].Molecular Biology and Evolution, 2007, 24: 1596—1599
[20] Saitou N, Nei M. The neighbor-joining method: a newmethod for reconstructing phylogenetic trees [J]. MolecularBiology and Evolution, 1987, 4(4): 406—425
[21] Kocher T D, Stepien C A. Molecular Systematics of Fishes[M]. San Diego: Academic Press. 1997, 113—128
[22] Bowen B W, Bass A L, Rocha L A, et al. Phylogeography ofthe trumpetfishes (Aulostomus): ring species complex on aglobal scale [J]. Evolution, 2001, 55: 1029—1039
[23] Yuan C M, Qin A L, Liu R H. On the classification of theanchovies, Coilia, from the lower Yangtze River and thesoutheast coast of China [J]. Journal of Nanjing University,1980, (3): 67—82 [袁传宓, 秦安舲, 刘仁华. 关于长江中下游及东南沿海各省的鲚属鱼类种下分类地位的探讨.南京大学学报, 1980, (3): 67—82]
[24] Liu W B. Biochemical and morphological comparison andinterspecific relationships of four species of the genus Coiliain China [J]. Oceanologia et Limnologia Sinica, 1995, 26(5):558—565 [刘文斌. 中国鲚属4 种鱼的生化和形态比较及其系统发育的研究. 海洋与湖沼, 1995, 26(5): 558—565]
[25] Tang W Q, Hu X L, Yang J Q. Species validities of Coiliabrachygnathus and C. nasus taihuensis based on sequencevariations of complete mtDNA control region [J]. BiodiversityScience, 2007, 15(3): 224—231 [唐文乔, 胡雪莲, 杨金权. 从线粒体控制区全序列变异看短颌鲚和湖鲚的物种有效性. 生物多样性, 2007, 15(3): 224—231]
[26] Wang Y Q, Xu Q S, Peng X K, et al. Taxonomic status ofamphioxus Branchiostoma belcheri in Xiamen Beach estimatedby homologous sequence of Cyt b gene [J]. Acta ZoologiaclSinica, 2004, 50(2): 202—208 [王义权, 许群山,彭宣克, 等. 通过Cyt b 基因同源序列比较评估厦门文昌鱼的分类学地位. 动物学报, 2004, 50(2): 202—208]
[27] Bian X D, Zhang X M, Gao T X, et al. Morphological andgenetic identification of Japanese halfbeak (Hyporhamphussajori) eggs [J]. Journal of Fisheries of China, 2008, 32(3):342—352 [卞晓东, 张秀梅, 高天翔, 等. 沙氏下鲢鱼卵的形态学及遗传学鉴别. 水产学报, 2008, 32(3): 342—352]
[28] Jiang R J, Zhong J S, Zhang D L, et al. Species compositionand diversity of fish larvae and juveniles in the surf zone ofthe Yangtze River Estuary [J]. Zoological Research, 2008,29(3): 297—304 [蒋日进, 钟俊生, 张冬良, 等. 长江口沿岸碎波带仔稚鱼的种类组成及其多样性特征. 动物学研究, 2008, 29(3): 297—304]
[29] Ge K K, Zhong J S, Wu M Q, et al. Distribution and abundanceof larval Japanese grenadier anchovy Coilia nasus inthe surf zone of the Yangtze River estuary [J]. Journal ofFishery Sciences, 2009, 16(6): 923—930 [葛珂珂, 钟俊生,吴美琴, 等. 长江口沿岸碎波带刀鲚仔稚鱼的数量分布.中国水产科学, 2009, 16(6): 923—930]
[30] Yang D L, Wu G Z, Sun J R. The investigation of pelagiceggs, larvae and juveniles of fishes at the mouth of theChangjiang River and adjacent areas [J]. Oceanologia etLimnologia Sinica, 1990, 21(4): 346—355 [杨东莱, 吴光宪,孙继仁. 长江口及其邻近海区的浮性鱼卵和仔稚鱼的生态研究. 海洋与湖沼, 1990, 21(4): 346—355]
[31] Zhu X H, Liu D, Sha X S. Relationship between communitystructure of ichthyoplankton and environmental factors in theestuary of Changjiang River in spring [J]. Studia MarinaSinica, 2002, 44: 169—178 [朱鑫华, 刘栋, 沙学绅. 长江口春季鱼类浮游生物群落结构与环境因子的关系. 海洋科学集刊, 2002, 44: 169—178]
[32] Jiang M, Shen X Q, Chen L F. Relationship between withabundance distribution of fish eggs, larvae and environmentalfactors in the Changjiang Estuary and vicinity watersin spring [J]. Marine Environmental Science, 2006, 26(2):37—44 [蒋玫, 沈新强, 陈莲芳. 长江口及邻近水域春季鱼卵仔鱼分布与环境因子的关系. 海洋环境科学, 2006,26(2): 37—44]
[33] Han L, Dai X J, Yang H, et al. Investigation on the resourcesstatus of fish larvae and juveniles in Yangtze Estuary Area [J].Journal of Anhui Agriculture Sciences, 2009, 37(13): 6023—6026 [韩璐, 戴小杰, 杨红, 等. 长江河口区仔稚鱼资源现状的调查. 安徽农业科学, 2009, 37(13): 6023—6026]
-
期刊类型引用(16)
1. 董微微,杨佐斌,谢意军,乔晔,邵科,熊美华,汪鄂洲,余丹,朱滨. 拉萨裂腹鱼在藏木和加查鱼道基于基因交流的过坝需求及其受到的遗传选择. 长江流域资源与环境. 2023(04): 783-796 . 百度学术
2. 倪健夫,郭弘艺,唐文乔,刘东,张亚,沈林宏. 长江靖江段沿岸凤鲚丰度的时间格局及生物学研究. 海洋渔业. 2020(05): 560-570 . 百度学术
3. 陈治,张岩,韩志强,宋娜,高天翔. Morphological characters and DNA barcoding of Syngnathus schlegeli in the coastal waters of China. Journal of Oceanology and Limnology. 2018(02): 537-547 . 百度学术
4. 孙超,刘洪波,姜涛,轩中亚,杨健. 不同鲚属鱼类Cyt b和D-loop序列的比较及其判别早期生活史个体的潜力分析. 渔业科学进展. 2018(05): 27-35 . 百度学术
5. 刘守海,秦玉涛,刘材材,季晓,张昊飞. DNA条形码技术在仔稚鱼鉴定中的实践. 海洋开发与管理. 2017(02): 92-95 . 百度学术
6. 王晓梅,朱国利,唐文乔. 刀鲚嗅觉受体基因MOR-51I2克隆、序列分析及组织表达. 水生生物学报. 2017(01): 33-42 . 本站查看
7. 张涛,王焕焕,毕学娟,宋超,赵峰,庄平. 长江口凤鲚仔稚鱼不同发育阶段矢耳石生长. 中国水产科学. 2017(06): 1315-1322 . 百度学术
8. 刘青,左中原,王志涛,王伟伟,宋晶,吕丽华. 山西沁河乌苏里拟鲿形态、分子鉴定及与黑龙江群体比较. 水生态学杂志. 2016(02): 76-82 . 百度学术
9. 毕雪娟,张涛,冯广朋,王妤,张婷婷,高宇,庄平. 长江口凤鲚个体生殖力的研究. 海洋渔业. 2015(03): 223-232 . 百度学术
10. 方亚璐,KAI Yoshiaki,YANAGIMOTO Takashi,宋娜,高天翔. A new record of Sebastes koreanus from China based on morphological characters and DNA barcoding. Chinese Journal of Oceanology and Limnology. 2015(03): 590-596 . 百度学术
11. 孙超,刘洪波,姜涛,杨健. 凤鲚ITS1与Cyt b基因序列的比较研究. 水产科学. 2013(09): 536-540 . 百度学术
12. 刘凯,徐东坡,段金荣,张敏莹,方弟安,周彦锋,施炜纲. 三峡蓄水后长江口凤鲚汛期生物学特征及捕捞量变动. 长江流域资源与环境. 2013(10): 1282-1288 . 百度学术
13. 孙超,刘洪波,姜涛,杨健. 分子生物学方法在鲚属鱼类遗传学研究中的应用. 江苏农业科学. 2013(01): 4-8 . 百度学术
14. 汪珂,刘凯,徐东坡,段金荣,张敏莹,陈永进,施炜纲. 鱼类早期资源研究进展. 江西农业大学学报. 2013(05): 1098-1107 . 百度学术
15. 李渊,宋娜,KHAN Fozia Siyal,柳本卓,高天翔. 银鲳形态特征与DNA条形码研究. 水产学报. 2013(11): 1601-1608 . 百度学术
16. 龚小玲,岳丽佳,崔忠凯,张晓懿. 鳗鲡属6种鱼类线粒体COⅠ和COⅡ基因序列分析和分类的有效性. 上海海洋大学学报. 2013(04): 524-530 . 百度学术
其他类型引用(12)
计量
- 文章访问数: 2282
- HTML全文浏览量: 0
- PDF下载量: 754
- 被引次数: 28