刀鲚和凤鲚的分子鉴定及其在仔鱼种类鉴定中的应用
MOLECULAR IDENTIFICATION OF COILIA ECTENES AND COILIA MYSTUS AND ITS APPLICATION ON LARVAL SPECIES
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摘要: 使用mtDNA Cyt b基因序列作为分子标记,基于400 bp左右的序列分析长颌鲚、短颌鲚及凤鲚成鱼的遗传关系。结果显示长颌鲚和短颌鲚间的遗传距离在0—0.5%之间;凤鲚与长颌鲚、短颌鲚的遗传距离分别在7.9%—8.9%和7.7%—8.6%之间。用Kimura双参数模型构建的NJ分子系统树显示,长颌鲚和短颌鲚不能形成彼此独立的分支,而是混合聚在一起形成一个分支;凤鲚则形成另一个分支。对长江口沿岸区域的鲚属仔鱼Cyt b基因片段序列分析发现,仔鱼个体之间的遗传距离为0—0.7%,仔鱼与长颌鲚、短颌鲚的遗传距离在0.1%—0.5%之间,而与凤鲚的遗传距离为8.3%—8.8%。在Kimura双参数模型构建的NJ分子系统树中,仔鱼与长颌鲚、短颌鲚聚为一类,而与凤鲚分开,表明分析的仔鱼样品不包括凤鲚个体。由于长江口一般没有短颌鲚分布,这些仔鱼应该都是长颌鲚。Abstract: Based on about 400 bp sequences, nucleotide sequences of Cytochrome (Cyt b) segments of mtDNA wereanalyzed for anadromous Coilia ectenes, freshwater resident C. ectenes and C. mystus in the Yangtze River, China, todevelop a reference data for larval species identification of genus Coilia. The genetic distance ranged from 0 to 0.5%between the anadromous and freshwater resident C. ectenes, 7.9% to 8.9% between C. mystus and the anadromous C.ectenes, and 7.7% to 8.6% between C. mystus and the freshwater resident C. ectenes. Neighbor-joining (NJ) moleculardendrogram using Kimura 2-parameter based on the sequences of Cyt b gene showed individuals of the anadromous andfreshwater resident C. ectenes mixed into a cluster, and those of C. mystus forming another cluster. Thus, larvae of C.mystus could be distinguished from that of C. ectenes through Cyt b analysis. Nucleotide sequences of Cyt b of larvae ofgenus Coilia collected from the Yangtze Estuary were analyzed, and compared to the reference established above. Geneticdistance ranged from 0.1% to 0.5% between the larvae and the anadromous and freshwater resident C. ectenes, and8.3% to 8.8% between the larvae and C. mystus. NJ molecular dendrogram showed that all the larvae and the anadromousand freshwater resident C. ectenes clustered together, while individuals of C. mystus forming a separate cluster.Thus, the larvae were not of C. mystus. As there is no freshwater resident C. ectenes inhabiting in the Estuary, the larvaeshould be of the anadromous C. ectenes.
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Keywords:
- Yangtze Estuary /
- Coilia ectenes /
- Coilia mystus /
- Larvae /
- Species identification /
- Cyt b gene
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